Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LAMP1 CRISPR/Cas9 KO Plasmid (h): sc-400120
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LAMP1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LAMP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LAMP1 Antibody (H4A3): sc-20011
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LAMP1 CRISPR/Cas9 KO Plasmid (h) | sc-400120 | 20 µg | $397.00 |
Overview
LAMP1 (lysosomal associated membrane protein 1) is a major type I membrane glycoprotein of late endosomes and lysosomes that helps maintain lysosomal integrity and participates in vesicular trafficking, endosome–lysosome fusion, and autophagy-lysosome flux. By contributing to lysosomal positioning, membrane protection, and degradative capacity, LAMP1 influences antigen processing, turnover of membrane proteins, and cellular responses to nutrient stress. Altered LAMP1 abundance or localization is frequently used as a readout of lysosomal biogenesis and stress responses, and it is linked to pathways relevant to neurodegeneration, infection, and cancer cell adaptation. LAMP1 is also commonly leveraged as a lysosomal marker in studies of organelle dynamics and lysosome-related signaling networks.
LAMP1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the LAMP1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the LAMP1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the LAMP1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LAMP1 protein expression.
This CRISPR knockout system enables efficient generation of LAMP1-deficient cell models for investigation of LAMP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting LAMP1 exon(s) critical for LAMP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple LAMP1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LAMP1 CRISPR/Cas9 KO Plasmid (h) and LAMP1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the LAMP1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LAMP1 HDR Plasmid (h) and LAMP1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by LAMP1 homology arms to support homology-directed repair at defined LAMP1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.