LAMP1 Antibody (H4A3): sc-20011

LAMP-1 Antibody (H4A3)20011 is a mouse monoclonal IgG1 kappa light chain antibody that detects LAMP-1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). LAMP-1 (H4A3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LAMP-1, also known as CD107a, is a glycosylated type I membrane protein that plays a crucial role in maintaining lysosomal function and integrity. LAMP-1 is primarily located on the lysosomal membrane, where LAMP-1 contributes to the regulation of lysosomal pH and protects lysosomal membranes from autodigestion. This localization is vital for cellular homeostasis, as lysosomes are responsible for degrading and recycling cellular waste, and any dysfunction in LAMP-1 can lead to lysosomal storage disorders. LAMP-1 is expressed on the surface of thrombin-activated platelets, suggesting LAMP-1′s involvement in the adhesive and prothrombotic properties of these cells, which is particularly important in the context of wound healing and inflammation. Expression levels of LAMP-1 and LAMP-2 are often elevated in various pathological conditions, highlighting their potential as biomarkers for disease states.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

LAMP1 Antibody (H4A3) References:

1. The tyrosine motifs of Lamp 1 and LAP determine their direct and indirect targetting to lysosomes.  |  Obermüller, S., et al. 2002. J Cell Sci. 115: 185-94. PMID: 11801736
2. Disturbed cholesterol traffic but normal proteolytic function in LAMP-1/LAMP-2 double-deficient fibroblasts.  |  Eskelinen, EL., et al. 2004. Mol Biol Cell. 15: 3132-45. PMID: 15121881
3. Identification and characterization of LAMP-1 as an activation-dependent platelet surface glycoprotein.  |  Febbraio, M. and Silverstein, RL. 1990. J Biol Chem. 265: 18531-7. PMID: 2211717
4. Lysosome-associated protein 1 (LAMP-1) and lysosome-associated protein 2 (LAMP-2) in a larger family carrier of Fabry disease.  |  Pereira, EM., et al. 2014. Gene. 536: 118-22. PMID: 24334114
5. A critical role of LAMP-1 in avian reovirus P10 degradation associated with inhibition of apoptosis and virus release.  |  Wu, H., et al. 2016. Arch Virol. 161: 899-911. PMID: 26744063
6. The lysosomal membrane glycoproteins Lamp-1 and Lamp-2 are present in mobilizable organelles, but are absent from the azurophil granules of human neutrophils.  |  Dahlgren, C., et al. 1995. Biochem J. 311 (Pt 2): 667-74. PMID: 7487911
7. Lysosome-associated membrane proteins h-LAMP1 (CD107a) and h-LAMP2 (CD107b) are activation-dependent cell surface glycoproteins in human peripheral blood mononuclear cells which mediate cell adhesion to vascular endothelium.  |  Kannan, K., et al. 1996. Cell Immunol. 171: 10-9. PMID: 8660832
8. Expression of Lamp-1 and Lamp-2 and their interactions with galectin-3 in human tumor cells.  |  Sarafian, V., et al. 1998. Int J Cancer. 75: 105-11. PMID: 9426697
9. Induction of melanogenesis during the various melanoma growth phases and the role of tyrosinase, lysosome-associated membrane proteins, and p90 calnexin in the melanogenesis cascade.  |  Salopek, TG. and Jimbow, K. 1996. J Investig Dermatol Symp Proc. 1: 195-202. PMID: 9627716
10. Sorting of lysosomal membrane glycoproteins lamp-1 and lamp-2 into vesicles distinct from mannose 6-phosphate receptor/gamma-adaptin vesicles at the trans-Golgi network.  |  Karlsson, K. and Carlsson, SR. 1998. J Biol Chem. 273: 18966-73. PMID: 9668075
11. Evaluation of the lysosome-associated membrane protein LAMP-2 as a marker for lysosomal storage disorders.  |  Hua, CT., et al. 1998. Clin Chem. 44: 2094-102. PMID: 9761240

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-LAMP1 Antibody (H4A3) has 518 citations in a variety of scientific publications.

Primary antibodies like Anti-LAMP1 Antibody (H4A3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

LAMP1 Antibody (H4A3) Specifications

Alternate Product Name: LAMP1

Product Clone Name: H4A3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: LAMP-1

Epitope Species: human

Product Reactivity/Detection: LAMP-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: monkey

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 518 citations in various scientific publications

Available Conjugates/Forms for LAMP1 Antibody (H4A3): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin