LAMP1 Antibody (E-5): sc-17768

LAMP-1 Antibody (E-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects LAMP-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-LAMP-1 antibody (E-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LAMP-1, also known as CD107a, is a glycosylated type I membrane protein primarily located on the lysosomal membrane, where LAMP-1 plays a crucial role in maintaining lysosomal integrity and function. LAMP-1 is essential for the proper degradation of cellular waste and the recycling of cellular components, which is vital for cellular homeostasis. Additionally, LAMP-1 is involved in the process of cellular adhesion and contributes to tumor invasion and metastasis, highlighting LAMP-1′s importance in both normal physiology and disease states. LAMP-1 expression is notably increased in lysosomal storage disorders, indicating LAMP-1′s potential as a biomarker for these conditions. LAMP-1 monoclonal antibody (E-5) enables researchers to gain insights into the roles of lysosomal proteins in various biological processes and disease mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

LAMP1 Antibody (E-5) References:

1. LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells.  |  Grützkau, A., et al. 2004. Cytometry A. 61: 62-8. PMID: 15351990
2. Roles of LAMP-1 and LAMP-2 in lysosome biogenesis and autophagy.  |  Eskelinen, EL. 2006. Mol Aspects Med. 27: 495-502. PMID: 16973206
3. Fucosylation of LAMP-1 and LAMP-2 by FUT1 correlates with lysosomal positioning and autophagic flux of breast cancer cells.  |  Tan, KP., et al. 2016. Cell Death Dis. 7: e2347. PMID: 27560716
4. Lysosome-associated membrane proteins-1 and -2 (LAMP-1 and LAMP-2) assemble via distinct modes.  |  Terasawa, K., et al. 2016. Biochem Biophys Res Commun. 479: 489-495. PMID: 27663661
5. LAMP-1 gene is overexpressed in high grade glioma.  |  Sarafian, VS., et al. 2018. APMIS. 126: 657-662. PMID: 29920782
6. The Major Lysosomal Membrane Proteins LAMP-1 and LAMP-2 Participate in Differentiation of C2C12 Myoblasts.  |  Sakane, H. and Akasaki, K. 2018. Biol Pharm Bull. 41: 1186-1193. PMID: 30068868
7. CD107a+ (LAMP-1) Cytotoxic CD8+ T-Cells in Lupus Nephritis Patients.  |  Wiechmann, A., et al. 2021. Front Med (Lausanne). 8: 556776. PMID: 33834029
8. LAMP-1 Chimeric to HIV-1 p55Gag in the Immunization of Neonate Mice Induces an Early Germinal Center Formation and AID Expression.  |  Teixeira, FME., et al. 2022. Vaccines (Basel). 10: PMID: 36016134
9. Deciphering cross-species reactivity of LAMP-1 antibodies using deep mutational epitope mapping and AlphaFold.  |  Pruvost, T., et al. 2023. MAbs. 15: 2175311. PMID: 36797224

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-LAMP1 Antibody (E-5) has 94 citations in a variety of scientific publications.

Primary antibodies like Anti-LAMP1 Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

LAMP1 Antibody (E-5) Specifications

Alternate Product Name: LAMP1

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-228

Epitope Target: LAMP-1

Epitope Species: human

Product Reactivity/Detection: LAMP-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 94 citations in various scientific publications

Available Conjugates/Forms for LAMP1 Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred LAMP1 antibody is LAMP1 Antibody (H4A3): sc-20011.