Laminin α-2 Antibody (4H8-2): sc-59854
- Laminin α-2 Antibody (4H8-2) is a rat monoclonal IgG1, cited in 59 publications, provided at 200 µg/ml
- raised against native Laminin α-2 of mouse origin, with epitope mapping to the N-terminal domain
- Anti-Laminin alpha-2 Antibody (4H8-2) is recommended for detection of Laminin α-2 of mouse, rat and human origin by WB, IP, IF and IHC(P)
- Anti-Laminin alpha-2 Antibody (4H8-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Laminin α-2 Antibody (4H8-2). This work is in progress.
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Laminin α-2 Antibody (4H8-2) is a rat monoclonal IgG1 antibody that detects Laminin α-2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Laminin α-2 antibody (4H8-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Laminins, including Laminin α-2, are crucial structural components of basement membranes, which are specialized extracellular matrices that provide support and anchorage to cells. These basement membranes are not only found beneath epithelial layers but also envelop blood vessels, muscle, peripheral nerves, and adipose tissue, playing a vital role in maintaining tissue integrity and function. The unique structure of Laminin α-2, which consists of a heterotrimeric assembly of α, β, and γ chains, allows interaction with various cell surface receptors, including integrins and dystroglycan, facilitating cell adhesion and signaling. Mutations in the Laminin α-2 gene are linked to congenital muscular dystrophy in humans and dystrophia muscularis in mice, highlighting its significance in muscle development and pathology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Laminin α-2 Antibody (4H8-2) References:
- Role of laminin isoforms in glomerular structure. | Hansen, K. and Abrass, CK. 1999. Pathobiology. 67: 84-91. PMID: 10023136
- Clinical correlations in 16 patients with total or partial laminin alpha2 deficiency characterized using antibodies against 2 fragments of the protein. | Morandi, L., et al. 1999. Arch Neurol. 56: 209-15. PMID: 10025426
- Form and function: the laminin family of heterotrimers. | Colognato, H. and Yurchenco, PD. 2000. Dev Dyn. 218: 213-34. PMID: 10842354
- Transcriptional regulation of laminin gene expression. | Aberdam, D., et al. 2000. Microsc Res Tech. 51: 228-37. PMID: 11054873
- Laminin alpha 2 (merosin)-deficient muscular dystrophy and demyelinating neuropathy in two cats. | O'Brien, DP., et al. 2001. J Neurol Sci. 189: 37-43. PMID: 11535231
- Laminin-α2 Chain-Deficient Congenital Muscular Dystrophy: Pathophysiology and Development of Treatment. | Durbeej, M. 2015. Curr Top Membr. 76: 31-60. PMID: 26610911
- Laminin Alpha 2 Enhances the Protective Effect of Exosomes on Human iPsc-Derived Cardiomyocytes in an In Vitro Ischemia-Reoxygenation Model. | Mesquita, FCP., et al. 2024. Int J Mol Sci. 25: PMID: 38612582
- The laminin family. | Tryggvason, K. 1993. Curr Opin Cell Biol. 5: 877-82. PMID: 8240830
- Role of laminin in endothelial cell recognition and differentiation. | Schnaper, HW., et al. 1993. Kidney Int. 43: 20-5. PMID: 8433560
- Domains of laminin. | Engvall, E. and Wewer, UM. 1996. J Cell Biochem. 61: 493-501. PMID: 8806072
- Disruption of the lama2 gene in embryonic stem cells: laminin alpha 2 is necessary for sustenance of mature muscle cells. | Kuang, W., et al. 1998. Exp Cell Res. 241: 117-25. PMID: 9633519
- Laminin isoforms and epithelial development. | Ekblom, M., et al. 1998. Ann N Y Acad Sci. 857: 194-211. PMID: 9917842
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Laminin α-2 Antibody (4H8-2) | sc-59854 | 200 µg/ml | $322.00 | |||
Laminin α-2 Antibody (4H8-2) AC | sc-59854 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Laminin α-2 Antibody (4H8-2) HRP | sc-59854 HRP | 200 µg/ml | $322.00 | |||
Laminin α-2 Antibody (4H8-2) FITC | sc-59854 FITC | 200 µg/ml | $336.00 | |||
Laminin α-2 Antibody (4H8-2) PE | sc-59854 PE | 200 µg/ml | $349.00 | |||
Laminin α-2 Antibody (4H8-2) Alexa Fluor® 488 | sc-59854 AF488 | 200 µg/ml | $364.00 | |||
Laminin α-2 Antibody (4H8-2) Alexa Fluor® 546 | sc-59854 AF546 | 200 µg/ml | $364.00 | |||
Laminin α-2 Antibody (4H8-2) Alexa Fluor® 594 | sc-59854 AF594 | 200 µg/ml | $364.00 | |||
Laminin α-2 Antibody (4H8-2) Alexa Fluor® 647 | sc-59854 AF647 | 200 µg/ml | $364.00 | |||
Laminin α-2 Antibody (4H8-2) Alexa Fluor® 680 | sc-59854 AF680 | 200 µg/ml | $364.00 | |||
Laminin α-2 Antibody (4H8-2) Alexa Fluor® 790 | sc-59854 AF790 | 200 µg/ml | $364.00 |