Lamellipodin Antibody (H-5): sc-390050

Lamellipodin Antibody (H-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects Lamellipodin protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Lamellipodin (H-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Lamellipodin, also known as ras-associated and pleckstrin homology domains-containing protein 1 (RAPH1), is a 1302 amino acid protein that plays a crucial role in cellular dynamics by mediating localized membrane signals and facilitating lamellipodia and filopodia formation, essential for cell migration and tissue development. This peripheral membrane protein is particularly important in cancer biology, as RAPH1 is downregulated in breast and ovarian cancers, with significantly reduced expression in metastatic osteosarcomas compared to primary tumors. Additionally, Lamellipodin interacts with ENAH/VASP proteins, vital for actin filament dynamics, further underscoring RAPH1′s significance in cellular motility and signaling pathways. Lamellipodin expression is widespread, with highest levels found in brain, heart, ovary, and developing embryos, while RMO1 isoform is predominantly expressed in liver.

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Lamellipodin Antibody (H-5) References:

1. A gene encoding a putative GTPase regulator is mutated in familial amyotrophic lateral sclerosis 2.  |  Hadano, S., et al. 2001. Nat Genet. 29: 166-73. PMID: 11586298
2. Lamellipodin, an Ena/VASP ligand, is implicated in the regulation of lamellipodial dynamics.  |  Krause, M., et al. 2004. Dev Cell. 7: 571-83. PMID: 15469845
3. Altered expression and deletion of RMO1 in osteosarcoma.  |  Eppert, K., et al. 2005. Int J Cancer. 114: 738-46. PMID: 15609301
4. UNC-6/Netrin induces neuronal asymmetry and defines the site of axon formation.  |  Adler, CE., et al. 2006. Nat Neurosci. 9: 511-8. PMID: 16520734
5. UNC-6/netrin and SLT-1/slit guidance cues orient axon outgrowth mediated by MIG-10/RIAM/lamellipodin.  |  Quinn, CC., et al. 2006. Curr Biol. 16: 845-53. PMID: 16563765
6. MIG-10/lamellipodin and AGE-1/PI3K promote axon guidance and outgrowth in response to slit and netrin.  |  Chang, C., et al. 2006. Curr Biol. 16: 854-62. PMID: 16618541
7. The consensus coding sequences of human breast and colorectal cancers.  |  Sjöblom, T., et al. 2006. Science. 314: 268-74. PMID: 16959974
8. Lamellipodin proline rich peptides associated with native plasma butyrylcholinesterase tetramers.  |  Li, H., et al. 2008. Biochem J. 411: 425-32. PMID: 18076380
9. c-Abl, Lamellipodin, and Ena/VASP proteins cooperate in dorsal ruffling of fibroblasts and axonal morphogenesis.  |  Michael, M., et al. 2010. Curr Biol. 20: 783-91. PMID: 20417104
10. SrGAP3 interacts with lamellipodin at the cell membrane and regulates Rac-dependent cellular protrusions.  |  Endris, V., et al. 2011. J Cell Sci. 124: 3941-55. PMID: 22159416
11. A FAK-Cas-Rac-lamellipodin signaling module transduces extracellular matrix stiffness into mechanosensitive cell cycling.  |  Bae, YH., et al. 2014. Sci Signal. 7: ra57. PMID: 24939893
12. Fluorescence imaging of lamellipodin-mediated biomolecular condensates on solid supported lipid bilayer membranes.  |  Narayan, KB., et al. 2024. Methods Enzymol. 700: 33-48. PMID: 38971606

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Lamellipodin Antibody (H-5) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Lamellipodin Antibody (H-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Lamellipodin Antibody (H-5) Specifications

Alternate Product Name: Lamellipodin

Product Clone Name: H-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 91-240

Epitope Target: Lamellipodin

Epitope Species: human

Product Reactivity/Detection: Lamellipodin

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Lamellipodin Antibody (H-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin