Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LAIR-1 CRISPR/Cas9 KO Plasmid (h): sc-403542
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LAIR-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LAIR-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LAIR-1 Antibody (F-5): sc-398141
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LAIR-1 CRISPR/Cas9 KO Plasmid (h) | sc-403542 | 20 µg | $397.00 |
Overview
LAIR1 encodes leukocyte-associated immunoglobulin-like receptor 1 (LAIR-1), an inhibitory collagen receptor broadly expressed on immune cells, including T cells, B cells, NK cells, monocytes, and dendritic cells. Upon binding extracellular matrix collagens, LAIR-1 signals through ITIM motifs to recruit phosphatases such as SHP-1/2, attenuating antigen receptor and Fc receptor pathways and constraining activation, cytokine production, and cytotoxicity. This checkpoint-like function links tissue matrix cues to immune homeostasis and influences leukocyte trafficking and inflammatory thresholds. Dysregulated LAIR-1 signaling has been associated with altered immune regulation in autoimmunity, chronic inflammation, infection, and tumor-associated immune suppression, making it a useful node for mechanistic immunology studies.
LAIR-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the LAIR1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the LAIR1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the LAIR1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LAIR-1 protein expression.
This CRISPR knockout system enables efficient generation of LAIR1-deficient cell models for investigation of LAIR-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting LAIR1 exon(s) critical for LAIR-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple LAIR1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LAIR-1 CRISPR/Cas9 KO Plasmid (h) and LAIR-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the LAIR1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LAIR-1 HDR Plasmid (h) and LAIR-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by LAIR1 homology arms to support homology-directed repair at defined LAIR1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.