LAIR-1 Antibody (F-5): sc-398141

LAIR-1 Antibody (F-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects LAIR-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). LAIR-1 (F-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The leukocyte-associated Ig-like receptor-1, or LAIR-1, is a transmembrane glycoprotein predominantly expressed on human peripheral blood mononuclear leukocytes, playing a crucial role in immune regulation. Notably, LAIR-1 undergoes phosphorylation at tyrosine residues 233 and 263, which are essential for function. Upon activation, LAIR-1 recruits SHP-1, a phosphatase that serves as a negative regulator of cell signaling, particularly in hematopoietic cells. This interaction helps establish T cell receptor signaling thresholds, influencing both development and function of T lymphocytes. Furthermore, LAIR-1 engagement on myelomonocytic leukemic cell lines inhibits cell proliferation and induces programmed cell death, while LAIR-1 cross-linking on natural killer cells significantly dampens cytotoxic activity. LAIR-1 phosphorylation is likely mediated by Src family kinases, as evidenced by PP1 inhibitory effects on this process.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

LAIR-1 Antibody (F-5) References:

1. Identification and characterization of leukocyte-associated Ig-like receptor-1 as a major anchor protein of tyrosine phosphatase SHP-1 in hematopoietic cells.  |  Xu, Mj., et al. 2000. J Biol Chem. 275: 17440-6. PMID: 10764762
2. FDF03, a novel inhibitory receptor of the immunoglobulin superfamily, is expressed by human dendritic and myeloid cells.  |  Fournier, N., et al. 2000. J Immunol. 165: 1197-209. PMID: 10903717
3. Engagement of the leukocyte-associated Ig-like receptor-1 induces programmed cell death and prevents NF-kappaB nuclear translocation in human myeloid leukemias.  |  Poggi, A., et al. 2000. Eur J Immunol. 30: 2751-8. PMID: 11069054
4. Constitutive association of SHP-1 with leukocyte-associated Ig-like receptor-1 in human T cells.  |  Sathish, JG., et al. 2001. J Immunol. 166: 1763-70. PMID: 11160222
5. Comparison of LAIR-1 genetic pathways in murine vs human internal organs.  |  Sun, S., et al. 2014. Gene. 552: 140-5. PMID: 25234728
6. Evidence for C1q-mediated crosslinking of CD33/LAIR-1 inhibitory immunoreceptors and biological control of CD33/LAIR-1 expression.  |  Son, M., et al. 2017. Sci Rep. 7: 270. PMID: 28325905
7. Molecular basis of reduced LAIR1 expression in childhood severe malarial anaemia: Implications for leukocyte inhibitory signalling.  |  Achieng, AO., et al. 2019. EBioMedicine. 45: 278-289. PMID: 31257148
8. Structural basis of LAIR1 targeting by polymorphic Plasmodium RIFINs.  |  Xu, K., et al. 2021. Nat Commun. 12: 4226. PMID: 34244481
9. Adiponectin's globular domain inhibits T cell activation by interacting with LAIR-1.  |  Zhang, Y., et al. 2021. Biochem Biophys Res Commun. 573: 117-124. PMID: 34403808
10. Effects of LAIR-1 on hepatocellular carcinoma cell proliferation and invasion via PI3K-AKT-mTOR pathway regulation.  |  Zhou, T., et al. 2023. Immun Inflamm Dis. 11: e982. PMID: 37647449
11. Efficient delivery of the lncRNA LEF1-AS1 through the antibody LAIR-1 (CD305)-modified Zn-Adenine targets articular inflammation to enhance the treatment of rheumatoid arthritis.  |  Zhang, X., et al. 2023. Arthritis Res Ther. 25: 238. PMID: 38062469
12. LAIR-1, a novel inhibitory receptor expressed on human mononuclear leukocytes.  |  Meyaard, L., et al. 1997. Immunity. 7: 283-90. PMID: 9285412

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-LAIR-1 Antibody (F-5) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-LAIR-1 Antibody (F-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

LAIR-1 Antibody (F-5) Specifications

Alternate Product Name: LAIR-1

Product Clone Name: F-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 182-287

Epitope Target: LAIR-1

Epitope Species: human

Product Reactivity/Detection: LAIR-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for LAIR-1 Antibody (F-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin