Anti-L-Caldesmon Antibody (F-10) recommended for detection of L-Caldesmon of human origin and Caldesmon of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA

Anti-L-Caldesmon Antibody (F-10): sc-25339

Anti-L-Caldesmon Antibody (F-10) is rated 5.0 out of 5 by 2.
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Datasheets

    • Anti-L-Caldesmon Antibody (F-10) is a mouse monoclonal IgG1 κ L-Caldesmon antibody, cited in 4 publications, provided at 200 µg/ml
    • raised against amino acids 494-793 of L-caldesmon of human origin
    • L-Caldesmon Antibody (F-10) is recommended for detection of L-Caldesmon of human origin and Caldesmon of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-L-Caldesmon Antibody (F-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of L-Caldesmon (F-10): sc-25339.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) are the preferred secondary detection reagents for L-Caldesmon Antibody (F-10) for WB and IHC(P) applications. These reagents are now offered in bundles with L-Caldesmon Antibody (F-10) (see ordering information below).
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I am using L-Caldesmon (F-10): sc-25339 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Exceptional immunofluorescence cytoskeletal Exceptional immunofluorescence cytoskeletal staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-05-21
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