KV1.1 CRISPR/Cas9 KO Plasmid (h): sc-403806
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KV1.1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KV1.1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: KV1.1 Antibody (2D8): sc-293345
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KV1.1 CRISPR/Cas9 KO Plasmid (h) | sc-403806 | 20 µg | $397.00 |
Overview
KCNA1 encodes the human voltage-gated potassium channel subunit KV1.1, a pore-forming component that contributes to delayed rectifier K+ currents and sets membrane excitability. KV1.1 influences action potential repolarization, firing frequency, and synaptic transmission, linking channel activity to neuronal signaling and network stability. Through its control of resting membrane potential and excitability-dependent Ca2+ entry, KV1.1 intersects with pathways governing neurotransmitter release and activity-dependent gene expression. Genetic and functional perturbations of KCNA1 are associated with neurological phenotypes, including episodic ataxia and seizure susceptibility, making it a relevant target for mechanistic studies of channelopathy biology.
KV1.1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KCNA1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KCNA1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KCNA1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KV1.1 protein expression.
This CRISPR knockout system enables efficient generation of KCNA1-deficient cell models for investigation of KV1.1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KCNA1 exon(s) critical for KV1.1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KCNA1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KV1.1 CRISPR/Cas9 KO Plasmid (h) and KV1.1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KCNA1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KV1.1 HDR Plasmid (h) and KV1.1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KCNA1 homology arms to support homology-directed repair at defined KCNA1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.