Ku86 Antibody (N9C1): sc-56135

Ku86 Antibody (N9C1) is a mouse monoclonal IgG1 antibody that detects Ku86 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Ku86, also known as Ku80 or p80, is a crucial component of the Ku protein complex, which is localized in the nucleus and consists of two subunits: Ku70 (p70) and Ku86 (p86). Anti-Ku86 antibody (N9C1) recognizes a protein that plays a vital role in DNA repair and maintenance, particularly in the non-homologous end joining (NHEJ) pathway, which is essential for repairing double-strand breaks in DNA. Ku86 maintains genomic stability, as defects can lead to increased susceptibility to cancer and other genetic disorders. Additionally, Ku86 regulates transcription by interacting with various transcription factors and RNA polymerases, thereby influencing gene expression. Ku86′s ability to bind to DNA and facilitate repair protein recruitment underscores its significance in cellular responses to DNA damage, making anti-Ku86 antibody (N9C1) an invaluable tool for researchers studying DNA repair mechanisms and related pathways.

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Ku86 Antibody (N9C1) References:

1. Human colon carcinoma cell-line HCT116 transfected by antisense cDNA as a tool to study the Ku86 involvement in cell proliferation.  |  Sadji, Z., et al. 2000. Cell Signal. 12: 745-50. PMID: 11152960
2. Ku86 autoantigen related protein-1 transcription initiates from a CpG island and is induced by p53 through a nearby p53 response element.  |  Braastad, CD., et al. 2002. Nucleic Acids Res. 30: 1713-24. PMID: 11937624
3. An antisense oligonucleotide targeted to human Ku86 messenger RNA sensitizes M059K malignant glioma cells to ionizing radiation, bleomycin, and etoposide but not DNA cross-linking agents.  |  Belenkov, AI., et al. 2002. Cancer Res. 62: 5888-96. PMID: 12384553
4. Function of DNA-protein kinase catalytic subunit during the early meiotic prophase without Ku70 and Ku86.  |  Hamer, G., et al. 2003. Biol Reprod. 68: 717-21. PMID: 12604618
5. Regulation of telomere length and suppression of genomic instability in human somatic cells by Ku86.  |  Myung, K., et al. 2004. Mol Cell Biol. 24: 5050-9. PMID: 15143195
6. Expression of DNA-PKcs and Ku86, but not Ku70, differs between lymphoid malignancies.  |  Holgersson, A., et al. 2004. Exp Mol Pathol. 77: 1-6. PMID: 15215044
7. Heterozygous inactivation of human Ku70/Ku86 heterodimer does not affect cell growth, double-strand break repair, or genome integrity.  |  Uegaki, K., et al. 2006. DNA Repair (Amst). 5: 303-11. PMID: 16325483
8. Deficient Pms2, ERCC1, Ku86, CcOI in field defects during progression to colon cancer.  |  Nguyen, H., et al. 2010. J Vis Exp.. PMID: 20689513
9. Analysis of Lamin B1, Vimentin and Anti-Ku86 as Prospective Biomarkers of Hepatocellular Carcinoma in Patients with Hepatitis C Virus Infection.  |  Idriss, NK., et al. 2019. Cell Physiol Biochem. 52: 595-605. PMID: 30897324
10. Ku86 is not required for protection of signal ends or for formation of nonstandard V(D)J recombination products.  |  Han, JO., et al. 1997. Mol Cell Biol. 17: 2226-34. PMID: 9121473

The preferred Ku86 antibody is Ku86 Antibody (B-1): sc-5280.