Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Ksr-1 CRISPR/Cas9 KO Plasmid (h): sc-401768
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ksr-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Ksr-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Ksr-1 Antibody (E-5): sc-515924
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ksr-1 CRISPR/Cas9 KO Plasmid (h) | sc-401768 | 20 µg | $397.00 |
Overview
KSR1 encodes kinase suppressor of Ras 1 (Ksr-1), a scaffold protein that organizes components of the RAF–MEK–ERK/MAPK cascade to modulate signal amplitude, duration, and subcellular localization. By coordinating MAPK module assembly downstream of receptor tyrosine kinases and RAS, Ksr-1 contributes to regulation of proliferation, differentiation, and survival decisions. KSR1 activity and expression have been linked to altered MAPK pathway output in oncogenic signaling contexts, and it has also been implicated in immune and inflammatory signaling networks that intersect with ERK-dependent transcriptional programs. These features make KSR1 a useful node for dissecting pathway wiring, feedback control, and context-specific signal propagation in human cells.
Ksr-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KSR1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KSR1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KSR1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Ksr-1 protein expression.
This CRISPR knockout system enables efficient generation of KSR1-deficient cell models for investigation of Ksr-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KSR1 exon(s) critical for Ksr-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KSR1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Ksr-1 CRISPR/Cas9 KO Plasmid (h) and Ksr-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KSR1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Ksr-1 HDR Plasmid (h) and Ksr-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KSR1 homology arms to support homology-directed repair at defined KSR1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.