Ksr-1 Antibody (E-5): sc-515924

Ksr-1 Antibody (E-5) is a mouse monoclonal IgM kappa antibody that detects Ksr-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Ksr-1 antibody (E-5) is available as a non-conjugated form, playing a crucial role in studying Ras signaling pathways. Ksr-1 (E-5) antibody recognizes kinase suppressor of Ras, primarily located in the cytoplasm and essential for proper functioning of Ras signaling cascade, which is vital for cell growth, differentiation, and survival. Cytoplasmic localization enables Ksr-1 to interact with key signaling molecules, such as Raf-1, which becomes activated by Ras and initiates downstream signaling pathways. Ksr-1 (E-5) monoclonal antibody helps researchers study interactions between Ksr-1 and Raf-1, critical for signal transduction promoting cellular responses to growth factors and other stimuli. Understanding Ksr-1′s cellular location and function provides insights into various physiological processes and potential implications in diseases where Ras signaling is dysregulated, such as cancer.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Ksr-1 Antibody (E-5) References:

1. Expression of kinase suppressor of Ras in the normal adult and embryonic mouse.  |  Giblett, SM., et al. 2002. Cell Growth Differ. 13: 307-13. PMID: 12133899
2. Protein kinase cascades in meiotic and mitotic cell cycle control.  |  Pelech, SL., et al. 1990. Biochem Cell Biol. 68: 1297-330. PMID: 2085430
3. KSR1 is overexpressed in endometrial carcinoma and regulates proliferation and TRAIL-induced apoptosis by modulating FLIP levels.  |  Llobet, D., et al. 2011. Am J Pathol. 178: 1529-43. PMID: 21435442
4. VRK2 anchors KSR1-MEK1 to endoplasmic reticulum forming a macromolecular complex that compartmentalizes MAPK signaling.  |  Fernández, IF., et al. 2012. Cell Mol Life Sci. 69: 3881-93. PMID: 22752157
5. Signal transduction from membrane to cytoplasm: growth factors and membrane-bound oncogene products increase Raf-1 phosphorylation and associated protein kinase activity.  |  Morrison, DK., et al. 1988. Proc Natl Acad Sci U S A. 85: 8855-9. PMID: 3057494
6. Insulin-stimulated microtubule-associated protein kinase is phosphorylated on tyrosine and threonine in vivo.  |  Ray, LB. and Sturgill, TW. 1988. Proc Natl Acad Sci U S A. 85: 3753-7. PMID: 3287375
7. Praja2 suppresses the growth of gastric cancer by ubiquitylation of KSR1 and inhibiting MEK-ERK signal pathways.  |  Zhao, Z., et al. 2021. Aging (Albany NY). 13: 3886-3897. PMID: 33461174
8. Characterization of murine A-raf, a new oncogene related to the v-raf oncogene.  |  Huleihel, M., et al. 1986. Mol Cell Biol. 6: 2655-62. PMID: 3491291
9. KSR: a novel player in the RAS pathway.  |  Downward, J. 1995. Cell. 83: 831-4. PMID: 8521506
10. KSR, a novel protein kinase required for RAS signal transduction.  |  Therrien, M., et al. 1995. Cell. 83: 879-88. PMID: 8521512
11. The C. elegans ksr-1 gene encodes a novel Raf-related kinase involved in Ras-mediated signal transduction.  |  Sundaram, M. and Han, M. 1995. Cell. 83: 889-901. PMID: 8521513
12. Identification of a member of the MAPKKK family as a potential mediator of TGF-beta signal transduction.  |  Yamaguchi, K., et al. 1995. Science. 270: 2008-11. PMID: 8533096

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ksr-1 Antibody (E-5) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-Ksr-1 Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ksr-1 Antibody (E-5) Specifications

Alternate Product Name: KSR1

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 240-264

Epitope Target: Ksr-1

Epitope Species: human

Product Reactivity/Detection: Ksr-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for Ksr-1 Antibody (E-5): N/A