KSHV K8α Antibody (8C12G10G1): sc-57889

KSHV K8α Antibody (8C12G10G1) is a mouse monoclonal IgG2b kappa light chain antibody that detects KSHV K8 alpha protein of KSHV origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). KSHV K8α (8C12G10G1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. KSHV K8 alpha protein, also known as ORF 72, is a viral homolog of mammalian cyclin D and plays a crucial role in cell cycle regulation by interacting with retinoblastoma protein (Rb). This interaction can lead to cyclin-dependent kinases (cdks) activation that are resistant to p16INK4a inhibitory effects, thereby promoting cell proliferation. K8α expression is particularly significant during KSHV lytic phase, and cidofovir does not inhibit K8α activity. KSHV associates with Kaposi′s sarcoma and other lymphoproliferative disorders, especially in immunocompromised individuals, highlighting the importance of understanding how KSHV proteins, including K8α, contribute to oncogenesis and disease progression. KSHV K8α gene encodes a K-bZIP protein that interacts with tumor suppressor p53, effectively repressing transcriptional activity and preventing p53-mediated apoptosis, which is critical for infected cell survival and virus persistence.

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KSHV K8α Antibody (8C12G10G1) References:

1. Detection of Kaposi's sarcoma herpesvirus DNA sequences in multiple myeloma bone marrow stromal cells.  |  Chauhan, D., et al. 1999. Blood. 93: 1482-6. PMID: 10029574
2. Bone marrow and peripheral blood dendritic cells from patients with multiple myeloma are phenotypically and functionally normal despite the detection of Kaposi's sarcoma herpesvirus gene sequences.  |  Raje, N., et al. 1999. Blood. 93: 1487-95. PMID: 10029575
3. Kaposi's sarcoma-associated herpesvirus gene sequences are detectable at low copy number in primary amyloidosis.  |  Raje, N., et al. 2000. Amyloid. 7: 126-32. PMID: 10842716
4. The K-bZIP protein from Kaposi's sarcoma-associated herpesvirus interacts with p53 and represses its transcriptional activity.  |  Park, J., et al. 2000. J Virol. 74: 11977-82. PMID: 11090200
5. Mechanisms governing expression of the v-FLIP gene of Kaposi's sarcoma-associated herpesvirus.  |  Grundhoff, A. and Ganem, D. 2001. J Virol. 75: 1857-63. PMID: 11160684
6. The ORF45 protein of Kaposi's sarcoma-associated herpesvirus is associated with purified virions.  |  Zhu, FX. and Yuan, Y. 2003. J Virol. 77: 4221-30. PMID: 12634379
7. bZIP proteins of human gammaherpesviruses.  |  Sinclair, AJ. 2003. J Gen Virol. 84: 1941-1949. PMID: 12867624
8. Kaposi's sarcoma-associated herpesvirus K8beta is derived from a spliced intermediate of K8 pre-mRNA and antagonizes K8alpha (K-bZIP) to induce p21 and p53 and blocks K8alpha-CDK2 interaction.  |  Yamanegi, K., et al. 2005. J Virol. 79: 14207-21. PMID: 16254356
9. HHV-8 (KSHV) does not establish latency in prostate cancer cell lines.  |  Munker, R., et al. 1997. Prostate. 33: 286-8. PMID: 9397202

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-KSHV K8α Antibody (8C12G10G1) has 19 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

KSHV K8α Antibody (8C12G10G1) Specifications

Alternate Product Name: KSHV K8α

Product Clone Name: 8C12G10G1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: KSHV K8α

Epitope Species: KSHV

Product Reactivity/Detection: Kaposi′s sarcoma-associated herpes virus (KSHV) K8α

Species Reactivity/Detection: KSHV

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for KSHV K8α Antibody (8C12G10G1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin