KLF6 CRISPR/Cas9 KO Plasmid (h): sc-400888
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KLF6 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KLF6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: KLF6 Antibody (E-10): sc-365633
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KLF6 CRISPR/Cas9 KO Plasmid (h) | sc-400888 | 20 µg | $397.00 |
Overview
KLF6 encodes Krüppel-like factor 6, a zinc-finger transcription factor that binds GC-rich promoter elements to regulate gene programs controlling cell-cycle progression, differentiation, and tissue remodeling. It integrates signals from pathways such as TGF-β/SMAD and broader stress-responsive transcriptional networks to modulate genes involved in proliferation and extracellular matrix regulation. Altered KLF6 expression or function has been associated with dysregulated growth control and inflammatory or fibrotic processes, making it relevant for studying transcriptional rewiring in disease contexts. In human cell models, KLF6 serves as a node for probing how transcription factors coordinate phenotype changes through promoter occupancy and downstream gene expression.
KLF6 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KLF6 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KLF6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KLF6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KLF6 protein expression.
This CRISPR knockout system enables efficient generation of KLF6-deficient cell models for investigation of KLF6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KLF6 exon(s) critical for KLF6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KLF6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KLF6 CRISPR/Cas9 KO Plasmid (h) and KLF6 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KLF6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KLF6 HDR Plasmid (h) and KLF6 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KLF6 homology arms to support homology-directed repair at defined KLF6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.