KIR3.1 Antibody (A-4): sc-365457
- KIR3.1 Antibody (A-4) is a mouse monoclonal IgG1 κ KIR3.1 antibody provided at 200 µg/ml
- raised against amino acids 356-500 mapping within a C-terminal cytoplasmic domain of KIR3.1 of human origin
- KIR3.1 Antibody (A-4) is recommended for detection of KIR3.1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-KIR3.1 Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for KIR3.1 Antibody (A-4) for WB applications. These reagents are now offered in bundles with KIR3.1 Antibody (A-4) (see ordering information below).
KIR3.1 Antibody (A-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects KIR3.1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). KIR3.1 (A-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. KIR3.1 protein, also known as GIRK1, is a member of the G protein-coupled inwardly rectifying potassium channels, which play a crucial role in regulating neuronal excitability and neurotransmitter release. These channels are predominantly located in the soma, dendrites, and axons of neurons, particularly in key brain regions such as the olfactory bulb, hippocampus, neocortex, dentate gyrus, cerebellar cortex, and thalamus. Proper functioning of KIR3.1 is vital for maintaining the balance of potassium ions across the neuronal membrane, which is essential for action potential generation and synaptic transmission. Additionally, KIR3.1 channels are regulated by G protein beta-gamma subunits, which can either enhance or inhibit their activity depending on the specific dimer composition. Notably, KIR3.1 activity can be modulated by post-translational modifications such as tyrosine phosphorylation, which can lead to channel inactivation, highlighting the importance of these regulatory mechanisms in neuronal signaling and potential implications in neurological disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
KIR3.1 Antibody (A-4) References:
- TrkB activation by brain-derived neurotrophic factor inhibits the G protein-gated inward rectifier Kir3 by tyrosine phosphorylation of the channel. | Rogalski, SL., et al. 2000. J Biol Chem. 275: 25082-8. PMID: 10833508
- Activation and inhibition of G protein-coupled inwardly rectifying potassium (Kir3) channels by G protein beta gamma subunits. | Lei, Q., et al. 2000. Proc Natl Acad Sci U S A. 97: 9771-6. PMID: 10944236
- Activation of alpha 1-adrenoceptors modulates the inwardly rectifying potassium currents of mammalian atrial myocytes. | Braun, AP., et al. 1992. Pflugers Arch. 421: 431-9. PMID: 1361052
- G-protein-gated inward rectifier K+ channel proteins (GIRK1) are present in the soma and dendrites as well as in nerve terminals of specific neurons in the brain. | Ponce, A., et al. 1996. J Neurosci. 16: 1990-2001. PMID: 8604043
- Neurotensin and dopamine D2 activation oppositely regulate the same K+ conductance in rat midbrain dopaminergic neurons. | Farkas, RH., et al. 1997. Neurosci Lett. 231: 21-4. PMID: 9280158
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KIR3.1 Antibody (A-4) | sc-365457 | 200 µg/ml | $322.00 | |||
KIR3.1 Antibody (A-4): m-IgG Fc BP-HRP Bundle | sc-527369 | 200 µg Ab; 10 µg BP | $361.00 | |||
KIR3.1 Antibody (A-4): m-IgG1 BP-HRP Bundle | sc-532742 | 200 µg Ab; 20 µg BP | $361.00 | |||
KIR3.1 Antibody (A-4) AC | sc-365457 AC | 500 µg/ml, 25% agarose | $424.00 | |||
KIR3.1 Antibody (A-4) HRP | sc-365457 HRP | 200 µg/ml | $322.00 | |||
KIR3.1 Antibody (A-4) FITC | sc-365457 FITC | 200 µg/ml | $336.00 | |||
KIR3.1 Antibody (A-4) PE | sc-365457 PE | 200 µg/ml | $349.00 | |||
KIR3.1 Antibody (A-4) Alexa Fluor® 488 | sc-365457 AF488 | 200 µg/ml | $364.00 | |||
KIR3.1 Antibody (A-4) Alexa Fluor® 546 | sc-365457 AF546 | 200 µg/ml | $364.00 | |||
KIR3.1 Antibody (A-4) Alexa Fluor® 594 | sc-365457 AF594 | 200 µg/ml | $364.00 | |||
KIR3.1 Antibody (A-4) Alexa Fluor® 647 | sc-365457 AF647 | 200 µg/ml | $364.00 | |||
KIR3.1 Antibody (A-4) Alexa Fluor® 680 | sc-365457 AF680 | 200 µg/ml | $364.00 | |||
KIR3.1 Antibody (A-4) Alexa Fluor® 790 | sc-365457 AF790 | 200 µg/ml | $364.00 |