KIFC1 CRISPR/Cas9 KO Plasmid (h): sc-405597
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KIFC1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KIFC1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: KIFC1 Antibody (M-63): sc-100947
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KIFC1 CRISPR/Cas9 KO Plasmid (h) | sc-405597 | 20 µg | $397.00 |
Overview
KIFC1 encodes a C-terminal kinesin-14 family motor protein that moves toward microtubule minus ends and contributes to mitotic spindle organization, centrosome clustering, and faithful chromosome segregation. By regulating microtubule dynamics and spindle pole integrity, KIFC1 interfaces with cell-cycle control and mitotic stress responses that shape proliferative capacity and genome stability. Dysregulated KIFC1 activity has been associated with aberrant mitoses, aneuploidy, and altered survival of cells experiencing centrosome amplification. These features make KIFC1 a useful node for studying mechanisms of spindle assembly, chromosomal instability, and microtubule-based transport in human cells.
KIFC1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KIFC1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KIFC1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KIFC1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KIFC1 protein expression.
This CRISPR knockout system enables efficient generation of KIFC1-deficient cell models for investigation of KIFC1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KIFC1 exon(s) critical for KIFC1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KIFC1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KIFC1 CRISPR/Cas9 KO Plasmid (h) and KIFC1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KIFC1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KIFC1 HDR Plasmid (h) and KIFC1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KIFC1 homology arms to support homology-directed repair at defined KIFC1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.