Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
KIF20A CRISPR/Cas9 KO Plasmid (h): sc-403194
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KIF20A CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KIF20A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: KIF20A Antibody (D-3): sc-374508
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KIF20A CRISPR/Cas9 KO Plasmid (h) | sc-403194 | 20 µg | $397.00 |
Overview
KIF20A (also known as MKlp2) encodes a kinesin-like motor protein that associates with microtubules to coordinate mitotic progression, central spindle assembly, and cytokinesis. It supports proper chromosome segregation and midbody formation through regulation of microtubule dynamics and spindle checkpoint–linked processes. KIF20A activity connects to cell-cycle control pathways governing G2/M transition and mitotic exit, and its dysregulation is frequently investigated in contexts of aberrant proliferation and aneuploidy in human disease. As a mitosis-associated motor, KIF20A is widely used as a functional node for studying cytoskeletal remodeling, division plane positioning, and proliferative signaling dependencies in cancer biology models.
KIF20A CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KIF20A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KIF20A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KIF20A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KIF20A protein expression.
This CRISPR knockout system enables efficient generation of KIF20A-deficient cell models for investigation of KIF20A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KIF20A exon(s) critical for KIF20A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KIF20A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KIF20A CRISPR/Cas9 KO Plasmid (h) and KIF20A CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KIF20A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KIF20A HDR Plasmid (h) and KIF20A HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KIF20A homology arms to support homology-directed repair at defined KIF20A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.