Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
KID CRISPR/Cas9 KO Plasmid (h): sc-408134
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KID CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KID genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: KID Antibody (B-9): sc-390640
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KID CRISPR/Cas9 KO Plasmid (h) | sc-408134 | 20 µg | $397.00 |
Overview
KIF22 encodes the chromokinesin KID, a microtubule-based motor that binds chromatin and generates polar ejection forces required for accurate chromosome congression and spindle dynamics during mitosis. KID activity integrates with kinetochore–microtubule attachments and the spindle assembly checkpoint to maintain proper chromosome alignment and segregation. Dysregulation of these processes contributes to chromosomal instability and aneuploidy, linking KIF22-dependent mitotic control to cancer-relevant cell cycle phenotypes and broader genome maintenance pathways.
KID CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KIF22 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KIF22 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KIF22 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KID protein expression.
This CRISPR knockout system enables efficient generation of KIF22-deficient cell models for investigation of KID signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KIF22 exon(s) critical for KID function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KIF22 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KID CRISPR/Cas9 KO Plasmid (h) and KID CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KIF22 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KID HDR Plasmid (h) and KID HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KIF22 homology arms to support homology-directed repair at defined KIF22 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.