KID Antibody (B-9): sc-390640
- KID Antibody (B-9) is a mouse monoclonal IgG2a κ KID antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 42-321 mapping near the N-terminus of KID of human origin
- KID Antibody (B-9) is recommended for detection of KID of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-KID Antibody (B-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for KID Antibody (B-9) for WB and IHC(P) applications. These reagents are now offered in bundles with KID Antibody (B-9) (see ordering information below).
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KID Antibody (B-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects KID protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-KID antibody (B-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. KID protein, also known as kinesin-like DNA-binding protein, plays a crucial role in cellular processes, particularly in spindle formation and chromosome movement during mitosis and meiosis, by binding to microtubules and DNA. KID (B-9) antibody recognizes a unique structure featuring a kinesin-like motor domain in the N-terminal half, which is essential for movement along microtubules, while the C-terminal helix-hairpin-helix DNA-binding domain allows interaction with DNA, highlighting dual functionality in both mitotic spindle dynamics and genomic stability. KID protein′s subcellular localization varies significantly during cell division, which underscores importance in ensuring accurate chromosome segregation and maintaining cellular integrity.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
KID Antibody (B-9) References:
- The Xenopus chromokinesin Xkid is essential for metaphase chromosome alignment and must be degraded to allow anaphase chromosome movement. | Funabiki, H. and Murray, AW. 2000. Cell. 102: 411-24. PMID: 10966104
- SIAH-1 interacts with alpha-tubulin and degrades the kinesin Kid by the proteasome pathway during mitosis. | Germani, A., et al. 2000. Oncogene. 19: 5997-6006. PMID: 11146551
- The human chromokinesin Kid is a plus end-directed microtubule-based motor. | Yajima, J., et al. 2003. EMBO J. 22: 1067-74. PMID: 12606572
- The second microtubule-binding site of monomeric kid enhances the microtubule affinity. | Shiroguchi, K., et al. 2003. J Biol Chem. 278: 22460-5. PMID: 12692123
- Importin-beta and the small guanosine triphosphatase Ran mediate chromosome loading of the human chromokinesin Kid. | Tahara, K., et al. 2008. J Cell Biol. 180: 493-506. PMID: 18268099
- Kid, a novel kinesin-like DNA binding protein, is localized to chromosomes and the mitotic spindle. | Tokai, N., et al. 1996. EMBO J. 15: 457-67. PMID: 8599929
- Human genes for KNSL4 and MAZ are located close to one another on chromosome 16p11.2. | Song, J., et al. 1998. Genomics. 52: 374-7. PMID: 9790757
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KID Antibody (B-9) | sc-390640 | 200 µg/ml | $322.00 | |||
KID Antibody (B-9): m-IgG Fc BP-HRP Bundle | sc-526180 | 200 µg Ab; 10 µg BP | $361.00 | |||
KID Antibody (B-9): m-IgG2a BP-HRP Bundle | sc-546523 | 200 µg Ab; 10 µg BP | $361.00 | |||
KID Antibody (B-9) AC | sc-390640 AC | 500 µg/ml, 25% agarose | $424.00 | |||
KID Antibody (B-9) HRP | sc-390640 HRP | 200 µg/ml | $322.00 | |||
KID Antibody (B-9) FITC | sc-390640 FITC | 200 µg/ml | $336.00 | |||
KID Antibody (B-9) PE | sc-390640 PE | 200 µg/ml | $349.00 | |||
KID Antibody (B-9) Alexa Fluor® 488 | sc-390640 AF488 | 200 µg/ml | $364.00 | |||
KID Antibody (B-9) Alexa Fluor® 546 | sc-390640 AF546 | 200 µg/ml | $364.00 | |||
KID Antibody (B-9) Alexa Fluor® 594 | sc-390640 AF594 | 200 µg/ml | $364.00 | |||
KID Antibody (B-9) Alexa Fluor® 647 | sc-390640 AF647 | 200 µg/ml | $364.00 | |||
KID Antibody (B-9) Alexa Fluor® 680 | sc-390640 AF680 | 200 µg/ml | $364.00 | |||
KID Antibody (B-9) Alexa Fluor® 790 | sc-390640 AF790 | 200 µg/ml | $364.00 |