Keratin 25 Antibody (B-2): sc-398320

Keratin 25 Antibody (B-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects Keratin 25 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Keratin 25 (B-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Keratin 25, also known as KRT25A or KRT25, is a 450 amino acid cytoplasmic protein that plays a crucial role in the structural integrity of epithelial tissues. KRT25 is essential for the proper assembly of type I and type II keratin protein complexes, which are vital for the formation of keratin intermediate filaments. These filaments provide mechanical support and resilience to cells, particularly in the skin and scalp, where KRT25 is strongly expressed. KRT25 exists as a heterotetramer composed of two type I and two type II keratins, and interacts specifically with Cytokeratin 6, highlighting KRT25′s importance in the formation of the inner root sheath of hair follicles. KRT25′s ability to form stable complexes is critical for maintaining the structural framework of epithelial cells, making KRT25 a significant target for studies related to skin and hair biology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Keratin 25 Antibody (B-2) References:

1. Characterization of a 300 kbp region of human DNA containing the type II hair keratin gene domain.  |  Rogers, MA., et al. 2000. J Invest Dermatol. 114: 464-72. PMID: 10692104
2. 'Hard' and 'soft' principles defining the structure, function and regulation of keratin intermediate filaments.  |  Coulombe, PA. and Omary, MB. 2002. Curr Opin Cell Biol. 14: 110-22. PMID: 11792552
3. Keratins of the human hair follicle.  |  Langbein, L. and Schweizer, J. 2005. Int Rev Cytol. 243: 1-78. PMID: 15797458
4. K25 (K25irs1), K26 (K25irs2), K27 (K25irs3), and K28 (K25irs4) represent the type I inner root sheath keratins of the human hair follicle.  |  Langbein, L., et al. 2006. J Invest Dermatol. 126: 2377-86. PMID: 16874310
5. Mutations in the helix termination motif of mouse type I IRS keratin genes impair the assembly of keratin intermediate filament.  |  Tanaka, S., et al. 2007. Genomics. 90: 703-11. PMID: 17920809
6. Sequence data and chromosomal localization of human type I and type II hair keratin genes.  |  Rogers, MA., et al. 1995. Exp Cell Res. 220: 357-62. PMID: 7556444
7. A new mutation in the type II hair cortex keratin hHb1 involved in the inherited hair disorder monilethrix.  |  Winter, H., et al. 1997. Hum Genet. 101: 165-9. PMID: 9402962
8. Characterization and chromosomal localization of human hair-specific keratin genes and comparative expression during the hair growth cycle.  |  Bowden, PE., et al. 1998. J Invest Dermatol. 110: 158-64. PMID: 9457912
9. A variable monilethrix phenotype associated with a novel mutation, Glu402Lys, in the helix termination motif of the type II hair keratin hHb1.  |  Winter, H., et al. 1998. J Invest Dermatol. 111: 169-72. PMID: 9665406

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Keratin 25 Antibody (B-2) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Keratin 25 Antibody (B-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Keratin 25 Antibody (B-2) Specifications

Alternate Product Name: Keratin 25

Product Clone Name: B-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 60-81

Epitope Target: Keratin 25

Epitope Species: human

Product Reactivity/Detection: Keratin 25

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Keratin 25 Antibody (B-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin