Kell Antibody (A-10): sc-377199
- Kell Antibody (A-10) is a mouse monoclonal IgG1 κ Kell antibody provided at 200 µg/ml
- raised against amino acids 170-269 mapping within an internal region of Kell of mouse origin
- Kell Antibody (A-10) is recommended for detection of Kell of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Kell Antibody (A-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for Kell Antibody (A-10) for WB and IHC(P) applications. This reagent is now offered in a bundle with Kell Antibody (A-10) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Kell Antibody (A-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects Kell protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Kell (A-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The KEL (CD238) gene encodes Kell protein, a type II transmembrane endopeptidase that plays a crucial role in regulating red blood cell membrane integrity and function. Kell protein is primarily expressed in erythroid tissues and testis, with lower levels found in brain and lymphoid tissues. Kell protein localization to Sertoli cells in testis and follicular dendritic cells in spleen and tonsil suggests a role in immune response and reproductive health. Kell protein is one of the major human surface antigens on red blood cells, where Kell protein is linked to XK protein by a single disulfide bond. XK protein absence, as seen in McLeod phenotype, is associated with clinical symptoms including nerve and muscle disorders and red cell acanthocytosis, highlighting Kell protein′s importance in both hematological and neurological health.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Kell Antibody (A-10) References:
- Molecular basis of the Kell-null phenotype: a mutation at the splice site of human KEL gene abolishes the expression of Kell blood group antigens. | Yu, LC., et al. 2001. J Biol Chem. 276: 10247-52. PMID: 11134029
- Transcriptional regulation of the KEL gene and Kell protein expression in erythroid and non-erythroid cells. | Camara-Clayette, V., et al. 2001. Biochem J. 356: 171-80. PMID: 11336649
- Molecular defects underlying the Kell null phenotype. | Lee, S., et al. 2001. J Biol Chem. 276: 27281-9. PMID: 11375401
- Kell expression on myeloid progenitor cells. | Wagner, T., et al. 2002. Leuk Lymphoma. 43: 479-85. PMID: 12002749
- Molecular cloning and primary structure of Kell blood group protein. | Lee, S., et al. 1991. Proc Natl Acad Sci U S A. 88: 6353-7. PMID: 1712490
- Organization of the gene encoding the human Kell blood group protein. | Lee, S., et al. 1995. Blood. 85: 1364-70. PMID: 7858266
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Kell Antibody (A-10) | sc-377199 | 200 µg/ml | $322.00 | |||
Kell Antibody (A-10): m-IgGκ BP-HRP Bundle | sc-551105 | 200 µg Ab; 40 µg BP | $361.00 | |||
Kell Antibody (A-10) AC | sc-377199 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Kell Antibody (A-10) HRP | sc-377199 HRP | 200 µg/ml | $322.00 | |||
Kell Antibody (A-10) FITC | sc-377199 FITC | 200 µg/ml | $336.00 | |||
Kell Antibody (A-10) PE | sc-377199 PE | 200 µg/ml | $349.00 | |||
Kell Antibody (A-10) Alexa Fluor® 488 | sc-377199 AF488 | 200 µg/ml | $364.00 | |||
Kell Antibody (A-10) Alexa Fluor® 546 | sc-377199 AF546 | 200 µg/ml | $364.00 | |||
Kell Antibody (A-10) Alexa Fluor® 594 | sc-377199 AF594 | 200 µg/ml | $364.00 | |||
Kell Antibody (A-10) Alexa Fluor® 647 | sc-377199 AF647 | 200 µg/ml | $364.00 | |||
Kell Antibody (A-10) Alexa Fluor® 680 | sc-377199 AF680 | 200 µg/ml | $364.00 | |||
Kell Antibody (A-10) Alexa Fluor® 790 | sc-377199 AF790 | 200 µg/ml | $364.00 |