KDEL receptor Antibody (KR-10): sc-57347

KDEL receptor Antibody (KR-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects the KDEL receptor protein of mouse, rat, human, and Drosophila origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-KDEL receptor antibody (KR-10) is available as a non-conjugated format. KDEL receptor plays a crucial role in the retention and retrieval of soluble proteins within the endoplasmic reticulum (ER), which is essential for maintaining cellular homeostasis. These proteins, characterized by the KDEL sequence at their carboxy terminus, are involved in various functions, including the formation of vesicles necessary for protein transport and the regulation of protein folding. KDEL receptor, also known as ERD2, recognizes these KDEL-tagged proteins as they pass through the Golgi apparatus and facilitates their retrograde transport back to the ER via COPI-coated vesicles. This process ensures that proteins meant to remain in the ER do not get secreted or mislocalized, thereby preventing potential cellular dysfunction. Additionally, the small GTPase ADP-ribosylation factor 1 (ARF1) interacts with KDEL receptor, which is significant for regulating vesicle transport and maintaining ER integrity. By recruiting a GTPase-activating protein from the cytosol, KDEL receptor helps inactivating ARF1, thus playing a pivotal role in the dynamic regulation of intracellular transport mechanisms.

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KDEL receptor Antibody (KR-10) References:

1. Identification of a novel mammalian endoplasmic reticulum-resident KDEL protein using an EST database motif search.  |  Kimata, Y., et al. 2000. Gene. 261: 321-7. PMID: 11167020
2. KDEL-cargo regulates interactions between proteins involved in COPI vesicle traffic: measurements in living cells using FRET.  |  Majoul, I., et al. 2001. Dev Cell. 1: 139-53. PMID: 11703931
3. Regulation of Golgi signaling and trafficking by the KDEL receptor.  |  Cancino, J., et al. 2013. Histochem Cell Biol. 140: 395-405. PMID: 23873287
4. Cargo binding promotes KDEL receptor clustering at the mammalian cell surface.  |  Becker, B., et al. 2016. Sci Rep. 6: 28940. PMID: 27353000
5. The KDEL receptor has a role in the biogenesis and trafficking of the epithelial sodium channel (ENaC).  |  Bikard, Y., et al. 2019. J Biol Chem. 294: 18324-18336. PMID: 31653700
6. KDEL receptor is a cell surface receptor that cycles between the plasma membrane and the Golgi via clathrin-mediated transport carriers.  |  Jia, J., et al. 2021. Cell Mol Life Sci. 78: 1085-1100. PMID: 32562023
7. Crosstalk between KDEL receptor and EGF receptor mediates cell proliferation and migration via STAT3 signaling.  |  Jia, J., et al. 2024. Cell Commun Signal. 22: 140. PMID: 38378560
8. The dynamic organisation of the secretory pathway.  |  Pelham, HR. 1996. Cell Struct Funct. 21: 413-9. PMID: 9118249
9. The KDEL receptor, ERD2, regulates intracellular traffic by recruiting a GTPase-activating protein for ARF1.  |  Aoe, T., et al. 1997. EMBO J. 16: 7305-16. PMID: 9405360
10. Identification of amino acids in the binding pocket of the human KDEL receptor.  |  Scheel, AA. and Pelham, HR. 1998. J Biol Chem. 273: 2467-72. PMID: 9442098
11. Modulation of intracellular transport by transported proteins: insight from regulation of COPI-mediated transport.  |  Aoe, T., et al. 1998. Proc Natl Acad Sci U S A. 95: 1624-9. PMID: 9465066