KCMF1 Antibody (A-3): sc-390051
- KCMF1 Antibody (A-3) is a mouse monoclonal IgG1 λ KCMF1 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 59-81 within an internal region of KCMF1 of human origin
- KCMF1 Antibody (A-3) is recommended for detection of KCMF1 of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and avian
- Anti-KCMF1 Antibody (A-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for KCMF1 Antibody (A-3) for WB and IHC(P) applications. This reagent is now offered in a bundle with KCMF1 Antibody (A-3) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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KCMF1 Antibody (A-3) is a mouse monoclonal IgG1 antibody that detects KCMF1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). KCMF1 (A-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. KCMF1, also known as potassium channel modulatory factor 1, plays a crucial role in cellular processes due to intrinsic E3 ubiquitin ligase activity, which is essential for ubiquitination of target proteins, thereby regulating their degradation and function. This post-translational modification is vital for maintaining cellular homeostasis and influencing various signaling pathways, particularly in epithelial morphogenesis, where KCMF1 is upregulated by basic fibroblast growth factor (FGF-2) in gastric cancer cells. KCMF1 consists of 381 amino acids and features one C2H2-type zinc finger and one ZZ-type zinc finger, which are critical for interaction with DNA and other proteins, facilitating a role in transcriptional regulation. KCMF1 is expressed in several tissues, including spleen, small intestine, ovary, peripheral blood, lung, kidney, and pancreas, while exhibiting lower expression levels in thymus, prostate, testis, colon, heart, brain, placenta, and liver, highlighting diverse functional implications across different biological systems.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
KCMF1 Antibody (A-3) References:
- Ubiquitin metabolism affects cellular response to volatile anesthetics in yeast. | Wolfe, D., et al. 1999. Mol Cell Biol. 19: 8254-62. PMID: 10567550
- Debt91, a putative zinc finger protein differentially expressed during epithelial morphogenesis. | Li, Z., et al. 2003. Biochem Biophys Res Commun. 306: 623-8. PMID: 12810064
- FIGC, a novel FGF-induced ubiquitin-protein ligase in gastric cancers. | Jang, JH. 2004. FEBS Lett. 578: 21-5. PMID: 15581609
- Suppression of KCMF1 by constitutive high CD99 expression is involved in the migratory ability of Ewing's sarcoma cells. | Kreppel, M., et al. 2006. Oncogene. 25: 2795-800. PMID: 16314831
- FGF signaling network in the gastrointestinal tract (review). | Katoh, M. and Katoh, M. 2006. Int J Oncol. 29: 163-8. PMID: 16773196
- Novel RING E3 ubiquitin ligases in breast cancer. | Burger, A., et al. 2006. Neoplasia. 8: 689-95. PMID: 16925951
- Evolution of C2H2-zinc finger genes revisited. | Thomas, JH. and Emerson, RO. 2009. BMC Evol Biol. 9: 51. PMID: 19261184
- Zinc fingers. | South, TL. and Summers, MF. 1990. Adv Inorg Biochem. 8: 199-248. PMID: 2119553
- Multiple genes encoding zinc finger domains are expressed in human T cells. | Thiesen, HJ. 1990. New Biol. 2: 363-74. PMID: 2288909
- KCMF1 regulates autophagy and ion channels' function in renal cell carcinoma: a future therapeutic target. | Singh, A., et al. 2023. J Cancer Res Clin Oncol. 149: 5617-5626. PMID: 36515749
- A Ubiquitination Cascade Regulating the Integrated Stress Response and Survival in Carcinomas. | Cervia, LD., et al. 2023. Cancer Discov. 13: 766-795. PMID: 36576405
- Disruption in networking of KCMF1 linked ubiquitin ligase impairs autophagy in CD8+ memory T cells of patients with renal cell carcinoma. | Singh, A., et al. 2023. Cancer Lett. 564: 216194. PMID: 37084875
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KCMF1 Antibody (A-3) | sc-390051 | 200 µg/ml | $316.00 | |||
KCMF1 Antibody (A-3): m-IgGλ BP-HRP Bundle | sc-523386 | 200 µg Ab, 40 µg BP | $354.00 | |||
KCMF1 Antibody (A-3) AC | sc-390051 AC | 500 µg/ml, 25% agarose | $416.00 | |||
KCMF1 Antibody (A-3) HRP | sc-390051 HRP | 200 µg/ml | $316.00 | |||
KCMF1 Antibody (A-3) FITC | sc-390051 FITC | 200 µg/ml | $330.00 | |||
KCMF1 Antibody (A-3) PE | sc-390051 PE | 200 µg/ml | $343.00 | |||
KCMF1 Antibody (A-3) Alexa Fluor® 488 | sc-390051 AF488 | 200 µg/ml | $357.00 | |||
KCMF1 Antibody (A-3) Alexa Fluor® 546 | sc-390051 AF546 | 200 µg/ml | $357.00 | |||
KCMF1 Antibody (A-3) Alexa Fluor® 594 | sc-390051 AF594 | 200 µg/ml | $357.00 | |||
KCMF1 Antibody (A-3) Alexa Fluor® 647 | sc-390051 AF647 | 200 µg/ml | $357.00 | |||
KCMF1 Antibody (A-3) Alexa Fluor® 680 | sc-390051 AF680 | 200 µg/ml | $357.00 | |||
KCMF1 Antibody (A-3) Alexa Fluor® 790 | sc-390051 AF790 | 200 µg/ml | $357.00 | |||
KCMF1 (A-3) Neutralizing Peptide | sc-390051 P | 100 µg/0.5 ml | $68.00 |