karyopherin β1 Antibody (H-7): sc-137016

karyopherin β1 Antibody (H-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects karyopherin beta 1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-karyopherin β1 antibody (H-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. karyopherin beta 1 protein plays a crucial role in nuclear transport of proteins, functioning as an adapter that facilitates translocation of cargo proteins across the nuclear envelope. This process maintains cellular function by regulating localization of proteins essential for gene expression, cell cycle progression, and signal transduction. karyopherin β1 interacts with karyopherin α1 to form a complex that recognizes and docks substrates at the nuclear pore complex, ensuring only properly tagged proteins are imported into the nucleus. karyopherin β1 has been implicated in transport of various viral proteins, such as Epstein-Barr virus nuclear antigen 1, highlighting significance in both cellular and viral biology. These interactions underscore importance in cellular homeostasis and potential implications in disease states where nuclear transport is disrupted.

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karyopherin β1 Antibody (H-7) References:

1. Regulation of nucleocytoplasmic transport by ADP-ribosylation: the emerging role of karyopherin-β1 mono-ADP-ribosylation by ARTD15.  |  Di Girolamo, M. 2015. Curr Top Microbiol Immunol. 384: 189-209. PMID: 25037261
2. The importin protein karyopherin-β1 regulates the mice fibroblast-like synoviocytes inflammation via facilitating nucleus transportation of STAT3 transcription factor.  |  Sun, C., et al. 2016. Biochem Biophys Res Commun. 471: 553-9. PMID: 26879143
3. Tumor-promoting properties of karyopherin β1 in melanoma by stabilizing Ras-GTPase-activating protein SH3 domain-binding protein 1.  |  Yang, F., et al. 2022. Cancer Gene Ther. 29: 1939-1950. PMID: 35902727
4. Importin subunit beta-1 mediates ERK5 nuclear translocation, and its inhibition synergizes with ERK5 kinase inhibitors in reducing cancer cell proliferation.  |  Lombardi, Z., et al. 2025. Mol Oncol. 19: 99-113. PMID: 38965815
5. Protein export from the nucleus requires the GTPase Ran and GTP hydrolysis.  |  Moroianu, J. and Blobel, G. 1995. Proc Natl Acad Sci U S A. 92: 4318-22. PMID: 7753805
6. Previously identified protein of uncertain function is karyopherin alpha and together with karyopherin beta docks import substrate at nuclear pore complexes.  |  Moroianu, J., et al. 1995. Proc Natl Acad Sci U S A. 92: 2008-11. PMID: 7892216
7. Ran binding domains promote the interaction of Ran with p97/beta-karyopherin, linking the docking and translocation steps of nuclear import.  |  Lounsbury, KM., et al. 1996. J Biol Chem. 271: 2357-60. PMID: 8576188
8. The binding site of karyopherin alpha for karyopherin beta overlaps with a nuclear localization sequence.  |  Moroianu, J., et al. 1996. Proc Natl Acad Sci U S A. 93: 6572-6. PMID: 8692858
9. Nuclear protein import: Ran-GTP dissociates the karyopherin alphabeta heterodimer by displacing alpha from an overlapping binding site on beta.  |  Moroianu, J., et al. 1996. Proc Natl Acad Sci U S A. 93: 7059-62. PMID: 8692944
10. Epstein-Barr virus nuclear antigen 1 forms a complex with the nuclear transporter karyopherin alpha2.  |  Fischer, N., et al. 1997. J Biol Chem. 272: 3999-4005. PMID: 9020106
11. Cloning and characterization of human karyopherin beta3.  |  Yaseen, NR. and Blobel, G. 1997. Proc Natl Acad Sci U S A. 94: 4451-6. PMID: 9114010
12. Karyopherin beta2 mediates nuclear import of a mRNA binding protein.  |  Bonifaci, N., et al. 1997. Proc Natl Acad Sci U S A. 94: 5055-60. PMID: 9144189

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-karyopherin β1 Antibody (H-7) has 32 citations in a variety of scientific publications.

Primary antibodies like Anti-karyopherin β1 Antibody (H-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

karyopherin β1 Antibody (H-7) Specifications

Alternate Product Name: KPNB1

Product Clone Name: H-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-300

Epitope Target: karyopherin β1

Epitope Species: human

Product Reactivity/Detection: karyopherin β1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 32 citations in various scientific publications

Available Conjugates/Forms for karyopherin β1 Antibody (H-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin