Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
karyopherin β2 CRISPR/Cas9 KO Plasmid (h): sc-403099
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- karyopherin β2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the karyopherin β2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: karyopherin β2 Antibody (F-6): sc-166127
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
karyopherin β2 CRISPR/Cas9 KO Plasmid (h) | sc-403099 | 20 µg | $397.00 |
Overview
TNPO1 encodes karyopherin β2 (transportin-1), a RanGTP-regulated import receptor that recognizes PY-type nuclear localization signals and mediates selective nuclear import of RNA-binding proteins and other cargo through the nuclear pore complex. By governing nucleocytoplasmic transport, karyopherin β2 influences RNA metabolism, stress granule dynamics, and transcriptional regulation, and connects to pathways such as the Ran cycle and nuclear pore transport machinery. Dysregulated transportin-dependent trafficking has been implicated in mechanisms relevant to protein mislocalization and aggregation as well as altered gene expression programs, providing a functional entry point for studying transport defects in disease-associated cellular states.
karyopherin β2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TNPO1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TNPO1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TNPO1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish karyopherin β2 protein expression.
This CRISPR knockout system enables efficient generation of TNPO1-deficient cell models for investigation of karyopherin β2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TNPO1 exon(s) critical for karyopherin β2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TNPO1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by karyopherin β2 CRISPR/Cas9 KO Plasmid (h) and karyopherin β2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TNPO1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by karyopherin β2 HDR Plasmid (h) and karyopherin β2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TNPO1 homology arms to support homology-directed repair at defined TNPO1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.