karyopherin β2 Antibody (F-6): sc-166127

karyopherin β2 Antibody (F-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects karyopherin beta 2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-karyopherin β2 antibody (F-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Karyopherin beta 2 plays a crucial role in the nuclear transport of proteins, specifically in the import of RNA-binding proteins into the nucleus, which is essential for regulating gene expression and maintaining cellular function. The ability of karyopherin beta 2 to facilitate the transport of these proteins underscores its importance in cellular processes, as disruptions in nuclear transport can lead to various diseases, including cancer and neurodegenerative disorders. karyopherin β2 monoclonal antibody (F-6) enables researchers to gain valuable insights into the mechanisms of nuclear transport and the implications of karyopherin beta 2 in health and disease.

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karyopherin β2 Antibody (F-6) References:

1. A proline-tyrosine nuclear localization signal (PY-NLS) is required for the nuclear import of fission yeast PAB2, but not of human PABPN1.  |  Mallet, PL. and Bachand, F. 2013. Traffic. 14: 282-94. PMID: 23279110
2. Nuclear localization signals for four distinct karyopherin-β nuclear import systems.  |  Soniat, M. and Chook, YM. 2015. Biochem J. 468: 353-62. PMID: 26173234
3. Nuclear-Import Receptors Reverse Aberrant Phase Transitions of RNA-Binding Proteins with Prion-like Domains.  |  Guo, L., et al. 2018. Cell. 173: 677-692.e20. PMID: 29677512
4. A new Karyopherin-β2 binding PY-NLS epitope of HNRNPH2 linked to neurodevelopmental disorders.  |  Gonzalez, A., et al. 2023. Structure. 31: 924-934.e4. PMID: 37279758
5. Protein export from the nucleus requires the GTPase Ran and GTP hydrolysis.  |  Moroianu, J. and Blobel, G. 1995. Proc Natl Acad Sci U S A. 92: 4318-22. PMID: 7753805
6. Previously identified protein of uncertain function is karyopherin alpha and together with karyopherin beta docks import substrate at nuclear pore complexes.  |  Moroianu, J., et al. 1995. Proc Natl Acad Sci U S A. 92: 2008-11. PMID: 7892216
7. Ran binding domains promote the interaction of Ran with p97/beta-karyopherin, linking the docking and translocation steps of nuclear import.  |  Lounsbury, KM., et al. 1996. J Biol Chem. 271: 2357-60. PMID: 8576188
8. The binding site of karyopherin alpha for karyopherin beta overlaps with a nuclear localization sequence.  |  Moroianu, J., et al. 1996. Proc Natl Acad Sci U S A. 93: 6572-6. PMID: 8692858
9. Nuclear protein import: Ran-GTP dissociates the karyopherin alphabeta heterodimer by displacing alpha from an overlapping binding site on beta.  |  Moroianu, J., et al. 1996. Proc Natl Acad Sci U S A. 93: 7059-62. PMID: 8692944
10. Epstein-Barr virus nuclear antigen 1 forms a complex with the nuclear transporter karyopherin alpha2.  |  Fischer, N., et al. 1997. J Biol Chem. 272: 3999-4005. PMID: 9020106
11. Cloning and characterization of human karyopherin beta3.  |  Yaseen, NR. and Blobel, G. 1997. Proc Natl Acad Sci U S A. 94: 4451-6. PMID: 9114010
12. Karyopherin beta2 mediates nuclear import of a mRNA binding protein.  |  Bonifaci, N., et al. 1997. Proc Natl Acad Sci U S A. 94: 5055-60. PMID: 9144189

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-karyopherin β2 Antibody (F-6) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-karyopherin β2 Antibody (F-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

karyopherin β2 Antibody (F-6) Specifications

Alternate Product Name: karyopherin β2

Product Clone Name: F-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-300

Epitope Target: karyopherin β2

Epitope Species: human

Product Reactivity/Detection: karyopherin β2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for karyopherin β2 Antibody (F-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred karyopherin β2/2B antibody is karyopherin β2/2B Antibody (A-11): sc-365179.