karyopherin α1 Antibody (187.1): sc-101292

karyopherin α1 Antibody (187.1) is a mouse monoclonal IgG1 kappa light chain antibody that detects karyopherin alpha 1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-karyopherin alpha 1 antibody (187.1) is available as a non-conjugated format. Karyopherin alpha 1 plays a crucial role in the nuclear transport system, specifically in recognizing and targeting proteins for nuclear import. This process maintains cellular function by regulating transport of proteins essential for gene expression, DNA repair, and cell cycle progression. Karyopherin alpha 1 primarily resides in the cytoplasm, binding to substrates before translocation through the nuclear pore complex. Substrate binding by karyopherin alpha 1 ensures only properly folded and functional proteins enter the nucleus, preventing cellular dysfunction. Karyopherin alpha 1 also interacts with various proteins, including karyopherin β1, which adapts to facilitate docking of the karyopherin α1/substrate complex to nucleoporins on the nuclear envelope. This intricate mechanism highlights karyopherin alpha 1′s importance in cellular transport and potential as a therapeutic target in nuclear transport dysregulation diseases.

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karyopherin α1 Antibody (187.1) References:

1. Identification of karyopherin α1 and α7 interacting proteins in porcine tissue.  |  Park, KE., et al. 2012. PLoS One. 7: e38990. PMID: 22720010
2. Karyopherin Alpha Proteins Regulate Oligodendrocyte Differentiation.  |  Laitman, BM., et al. 2017. PLoS One. 12: e0170477. PMID: 28107514
3. Acetylation regulates the nucleocytoplasmic distribution and oncogenic function of karyopherin alpha 2 in lung adenocarcinoma.  |  Feng, HP., et al. 2023. Biochem Biophys Res Commun. 659: 96-104. PMID: 37060831
4. Association of skeletal muscle karyopherin α1 and α2 with myogenesis and insulin action.  |  Karczewska-Kupczewska, M., et al. 2024. Pol Arch Intern Med. 134: PMID: 38980143
5. Protein export from the nucleus requires the GTPase Ran and GTP hydrolysis.  |  Moroianu, J. and Blobel, G. 1995. Proc Natl Acad Sci U S A. 92: 4318-22. PMID: 7753805
6. Previously identified protein of uncertain function is karyopherin alpha and together with karyopherin beta docks import substrate at nuclear pore complexes.  |  Moroianu, J., et al. 1995. Proc Natl Acad Sci U S A. 92: 2008-11. PMID: 7892216
7. Ran binding domains promote the interaction of Ran with p97/beta-karyopherin, linking the docking and translocation steps of nuclear import.  |  Lounsbury, KM., et al. 1996. J Biol Chem. 271: 2357-60. PMID: 8576188
8. The binding site of karyopherin alpha for karyopherin beta overlaps with a nuclear localization sequence.  |  Moroianu, J., et al. 1996. Proc Natl Acad Sci U S A. 93: 6572-6. PMID: 8692858
9. Nuclear protein import: Ran-GTP dissociates the karyopherin alphabeta heterodimer by displacing alpha from an overlapping binding site on beta.  |  Moroianu, J., et al. 1996. Proc Natl Acad Sci U S A. 93: 7059-62. PMID: 8692944
10. Epstein-Barr virus nuclear antigen 1 forms a complex with the nuclear transporter karyopherin alpha2.  |  Fischer, N., et al. 1997. J Biol Chem. 272: 3999-4005. PMID: 9020106
11. Cloning and characterization of human karyopherin beta3.  |  Yaseen, NR. and Blobel, G. 1997. Proc Natl Acad Sci U S A. 94: 4451-6. PMID: 9114010
12. Karyopherin beta2 mediates nuclear import of a mRNA binding protein.  |  Bonifaci, N., et al. 1997. Proc Natl Acad Sci U S A. 94: 5055-60. PMID: 9144189

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-karyopherin α1 Antibody (187.1) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-karyopherin α1 Antibody (187.1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

karyopherin α1 Antibody (187.1) Specifications

Alternate Product Name: karyopherin α1

Product Clone Name: 187.1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-539

Epitope Target: karyopherin α1

Epitope Species: human

Product Reactivity/Detection: karyopherin α1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for karyopherin α1 Antibody (187.1): N/A

The preferred karyopherin α1/6 antibody is karyopherin α1/6 Antibody (2D9): sc-101540.