karyopherin alpha 1/6 Antibody (2D9) recommended for detection of karyopherin α1 and karyopherin α6 of mouse, rat, human, hamster, bovine and monkey origin by WB, IP, IF and ELISA

karyopherin α1/6 Antibody (2D9): sc-101540

karyopherin α1/6 Antibody (2D9) is rated 5.0 out of 5 by 3.
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Datasheets

    • Anti-karyopherin α1/6 Antibody (2D9) is a rat monoclonal IgG2a karyopherin α1/6 antibody provided at 200 µg/ml
    • raised against full-length recombinant karyopherin α6 of mouse origin
    • Anti-karyopherin alpha 1/6 Antibody (2D9) is recommended for detection of karyopherin α1 and karyopherin α6 of mouse, rat, human, hamster, bovine and monkey origin by WB, IP, IF and ELISA
    • karyopherin alpha 1/6 Antibody (2D9) is available conjugated to agarose for IP; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of karyopherin α1/6 (2D9): sc-101540.

What is the recommended fixation method for immunofluorescence staining with karyopherin α1/6 (2D9): sc-101540 monoclonal antibody?

Asked by: TinTin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Clean data obtained in WB using non Clean data obtained in WB using non-transfected 293T, human karyopherin 1 transfected 293T and HeLa whole cell lysates -SCBT QC
Date published: 2015-05-03
Rated 5 out of 5 by from Great mono for western blotting; tested in HeLa Great mono for western blotting; tested in HeLa, K-562, Hep G2, Caki-1, ES-2, HuT 78 and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-04-11
Rated 5 out of 5 by from Produced beautiful immunofluorescence nuclear Produced beautiful immunofluorescence nuclear staining in formalin-fixed Hep G2 cells. -SCBT QC
Date published: 2015-03-08
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