KAP3 Antibody (D-6): sc-55598

KAP3 Antibody (D-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects KAP3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). KAP3 (D-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The mouse kinesin superfamily-associated protein 3 (KAP3) and human homolog KAP3A are globular proteins that play a crucial role as linkers for chromosome-associated proteins, facilitating proper organization and movement of chromosomes during cell division. KAP3 is primarily located in the cytoplasm, where KAP3 interacts with kinesin superfamily proteins, KIF3A and KIF3B, which are essential for transport of membranous organelles along axonal pathways. KAP3 presence in the cytoplasm is vital for maintaining cellular transport mechanisms and ensuring organelles are delivered to appropriate destinations, which is critical for neuronal function and overall cellular health. Additionally, mouse KAP3 can produce an alternative isoform, KAP3B, due to a stop codon insertion at the C-terminus, highlighting the complexity and regulatory potential. Initial studies of human homolog, also known as SMAP (Small G protein GDP dissociation stimulator-associated protein), have revealed KAP3′s role as an adaptor protein for Smg GDS and kinesin II, as well as a substrate for tyrosine phosphorylation by v-Src, further emphasizing KAP3′s multifaceted functions in cellular processes.

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KAP3 Antibody (D-6) References:

1. Left-right asymmetry and kinesin superfamily protein KIF3A: new insights in determination of laterality and mesoderm induction by kif3A-/- mice analysis.  |  Takeda, S., et al. 1999. J Cell Biol. 145: 825-36. PMID: 10330409
2. Immunolocalization of the heterotrimeric kinesin-related protein KRP(85/95) in the mitotic apparatus of sea urchin embryos.  |  Henson, JH., et al. 1995. Dev Biol. 171: 182-94. PMID: 7556895
3. KIF3A/B: a heterodimeric kinesin superfamily protein that works as a microtubule plus end-directed motor for membrane organelle transport.  |  Yamazaki, H., et al. 1995. J Cell Biol. 130: 1387-99. PMID: 7559760
4. Cloning and characterization of KAP3: a novel kinesin superfamily-associated protein of KIF3A/3B.  |  Yamazaki, H., et al. 1996. Proc Natl Acad Sci U S A. 93: 8443-8. PMID: 8710890
5. SMAP, an Smg GDS-associating protein having arm repeats and phosphorylated by Src tyrosine kinase.  |  Shimizu, K., et al. 1996. J Biol Chem. 271: 27013-7. PMID: 8900189
6. Complex formation of SMAP/KAP3, a KIF3A/B ATPase motor-associated protein, with a human chromosome-associated polypeptide.  |  Shimizu, K., et al. 1998. J Biol Chem. 273: 6591-4. PMID: 9506951

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-KAP3 Antibody (D-6) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-KAP3 Antibody (D-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

KAP3 Antibody (D-6) Specifications

Alternate Product Name: KAP3

Product Clone Name: D-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 494-793

Epitope Target: KAP3

Epitope Species: mouse

Product Reactivity/Detection: KAP3A and KAP3B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for KAP3 Antibody (D-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin