KRAS Antibody (F234): sc-30

K-Ras Antibody (F234) is a mouse monoclonal IgG2a kappa light chain antibody, specifically raised against recombinant K-Ras protein encompassing amino acids 54-189 of rat origin, and demonstrates reactivity with K-Ras proteins from mouse, rat, and human sources in applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). The K-Ras protein is a small GTPase belonging to the Ras family, playing a pivotal role in cellular signal transduction pathways that regulate cell proliferation, differentiation, and survival. K-Ras cycles between an active GTP-bound state and an inactive GDP-bound state, a process regulated by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). K-Ras is primarily localized to the inner surface of the plasma membrane, a location critical for function because K-Ras facilitates interactions with upstream activators and downstream effectors in signaling pathways. This membrane association is mediated by post-translational modifications, such as farnesylation and palmitoylation, which anchor K-Ras to the lipid bilayer. The precise cellular localization of K-Ras is essential for transmitting signals that control vital cellular processes, as mislocalization can disrupt normal signaling, leading to uncontrolled cell growth and contributing to oncogenesis. Mutations in the KRAS gene are among the most common in human cancers and are associated with poor prognosis. Understanding the localization and function of K-Ras is therefore crucial for cancer research and the development of targeted therapies. K-Ras (F234) antibody is an invaluable tool for researchers studying the expression, localization, and function of K-Ras in both normal physiology and disease states.

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KRAS Antibody (F234) References:

1. The dark side of Ras: regulation of apoptosis.  |  Cox, AD. and Der, CJ. 2003. Oncogene. 22: 8999-9006. PMID: 14663478
2. Ras signaling in prostate cancer progression.  |  Weber, MJ. and Gioeli, D. 2004. J Cell Biochem. 91: 13-25. PMID: 14689577
3. Human RAS superfamily proteins and related GTPases.  |  Colicelli, J. 2004. Sci STKE. 2004: RE13. PMID: 15367757
4. Oncogenic Ras in tumour progression and metastasis.  |  Giehl, K. 2005. Biol Chem. 386: 193-205. PMID: 15843165
5. Ras plasma membrane signalling platforms.  |  Hancock, JF. and Parton, RG. 2005. Biochem J. 389: 1-11. PMID: 15954863
6. Ras signaling on the Golgi.  |  Quatela, SE. and Philips, MR. 2006. Curr Opin Cell Biol. 18: 162-7. PMID: 16488589
7. Compartmentalized Ras/MAPK signaling.  |  Mor, A. and Philips, MR. 2006. Annu Rev Immunol. 24: 771-800. PMID: 16551266
8. Ras, PI(3)K and mTOR signalling controls tumour cell growth.  |  Shaw, RJ. and Cantley, LC. 2006. Nature. 441: 424-30. PMID: 16724053
9. Three distinct genes in human DNA related to the transforming genes of mammalian sarcoma retroviruses.  |  Wong-Staal, F., et al. 1981. Science. 213: 226-8. PMID: 6264598

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-KRAS Antibody (F234) has 459 citations in a variety of scientific publications.

Primary antibodies like Anti-KRAS Antibody (F234) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

KRAS Antibody (F234) Specifications

Alternate Product Name: KRAS

Product Clone Name: F234

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 54-189

Epitope Target: K-Ras

Epitope Species: rat

Product Reactivity/Detection: c-K-Ras and v-K-Ras

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 459 citations in various scientific publications

Available Conjugates/Forms for KRAS Antibody (F234): N/A

The preferred KRAS antibody is KRAS Antibody (B-12): sc-518124.