pan Ras Antibody (C-4): sc-166691

Ras Antibody (pan) (C-4) is a mouse monoclonal IgG2b kappa light chain antibody raised against human Ras proteins, crucial GTPases involved in cellular signal transduction. Anti-Ras antibody (C-4) recognizes all Ras isoforms (H-, K-, and N-Ras) and demonstrates broad species cross-reactivity, detecting Ras proteins in mouse, rat, and human samples through Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry on paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA) applications. Ras monoclonal antibody (C-4) is available in non-conjugated form and multiple conjugated forms including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, offering versatility for diverse experimental needs. Encoded by c-H-, c-K-, and N-Ras proto-oncogenes, these guanine nucleotide-binding proteins maintain widespread distribution throughout vertebrate cells, where Ras proteins serve as essential mediators transmitting signals from cell surface receptors to intracellular effectors. Ras protein function undergoes sophisticated regulation through interactions with GTPase-activating proteins (GAPs), though mutations at specific amino acid positions can lead to constitutive activation and oncogenic transformation. Through these diverse cellular functions, Ras (C-4) antibody provides researchers with a valuable tool for investigating signal transduction pathways and cancer biology mechanisms.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-pan Ras Antibody (C-4) has 43 citations in a variety of scientific publications.

Primary antibodies like Anti-pan Ras Antibody (C-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

pan Ras Antibody (C-4) Specifications

Alternate Product Name: pan Ras

Product Clone Name: C-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-189

Epitope Target: N-Ras

Epitope Species: human

Product Reactivity/Detection: N-Ras, H-Ras and K-Ras p21

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 43 citations in various scientific publications

Available Conjugates/Forms for pan Ras Antibody (C-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred KRAS antibody is KRAS Antibody (F234): sc-30.