Josephin-3 Antibody (B-10): sc-515158

Josephin-3 Antibody (B-10) is a mouse monoclonal IgG2b antibody that detects Josephin-3 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Josephin-3, also known as TATA box-binding protein-associated factor RNA polymerase I subunit D, is a crucial 278 amino acid transcription factor encoded by the human TAF1D gene. anti-Josephin-3 antibody (B-10) plays a vital role in studying the assembly of the SL1 complex, which is essential for the formation of the preinitiation complex necessary for RNA polymerase I-dependent transcription. The SL1 complex not only stabilizes the nucleolar transcription factor 1/UBTF on ribosomal DNA but also ensures proper cell cycle progression, particularly through G2/M phase-specific phosphorylations. The localization of Josephin-3 in the nucleus is significant, as this positioning allows for direct interaction with the transcription machinery, thereby influencing gene expression and cellular function. Impairments in the SL1 complex can lead to reduced levels of RNA polymerase I transcription, highlighting the importance of Josephin-3 in maintaining normal cellular processes. Josephin-3 monoclonal antibody (B-10) is expressed in most tissues, making this reagent a valuable tool for researchers studying transcription regulation and its implications in various biological contexts.

The preferred Josephin-3 antibody is Josephin-3 Antibody (G-12): sc-514821.