Josephin-3 Antibody (G-12): sc-514821
- Josephin-3 Antibody (G-12) is a mouse monoclonal IgM κ Josephin-3 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 261-275 at the C-terminus of Josephin-3 of human origin
- recommended for detection of Josephin-3 of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgGκ BP-HRP is the preferred secondary detection reagent for Josephin-3 Antibody (G-12) for WB applications. This reagent is now offered in a bundle with Josephin-3 Antibody (G-12) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Josephin-3 Antibody (G-12) is a mouse monoclonal IgM antibody that detects Josephin-3 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Josephin-3 antibody (G-12) is available as the non-conjugated form. Josephin-3, also known as TATA box-binding protein-associated factor RNA polymerase I subunit D, is a crucial 278 amino acid transcription factor encoded by the human TAF1D gene. Josephin-3 plays a vital role in the assembly of the SL1 complex, which is essential for the formation of the preinitiation complex necessary for RNA polymerase I-dependent transcription. The SL1 complex, which includes TAF1D, stabilizes the nucleolar transcription factor 1/UBTF on ribosomal DNA, thereby facilitating the transcription of ribosomal RNA. Disruption of the SL1 complex can lead to decreased levels of RNA polymerase I transcription, which is critical for ribosome biogenesis and overall cellular function. Additionally, TAF1D is involved in cell cycle regulation, exhibiting specific phosphorylations during the G2/M phase, and is predominantly localized in the nucleus, highlighting its importance in gene expression and cellular proliferation.
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Josephin-3 Antibody (G-12) References:
- TBP-associated factor 1 overexpression induces tolerance to Doxorubicin in confluent H9c2 cells by an increase in cdk2 activity and cyclin E expression. | Servant, N., et al. 2004. Mol Cell Biochem. 259: 71-81. PMID: 15124910
- TBP-TAF complex SL1 directs RNA polymerase I pre-initiation complex formation and stabilizes upstream binding factor at the rDNA promoter. | Friedrich, JK., et al. 2005. J Biol Chem. 280: 29551-8. PMID: 15970593
- A novel TBP-associated factor of SL1 functions in RNA polymerase I transcription. | Gorski, JJ., et al. 2007. EMBO J. 26: 1560-8. PMID: 17318177
- A functional genome-wide RNAi screen identifies TAF1 as a regulator for apoptosis in response to genotoxic stress. | Kimura, J., et al. 2008. Nucleic Acids Res. 36: 5250-9. PMID: 18684994
- Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. | Mayya, V., et al. 2009. Sci Signal. 2: ra46. PMID: 19690332
- Quantitative mass spectrometry of TATA binding protein-containing complexes and subunit phosphorylations during the cell cycle. | Pijnappel, WP., et al. 2009. Proteome Sci. 7: 46. PMID: 20034391
- Comparing transcriptional initiation by RNA polymerases I and III. | Geiduschek, EP. and Kassavetis, GA. 1995. Curr Opin Cell Biol. 7: 344-51. PMID: 7662364
- TBP-associated factors interact with DNA and govern species specificity of RNA polymerase I transcription. | Rudloff, U., et al. 1994. EMBO J. 13: 2611-6. PMID: 8013460
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Josephin-3 Antibody (G-12) | sc-514821 | 200 µg/ml | $322.00 | |||
Josephin-3 Antibody (G-12): m-IgGκ BP-HRP Bundle | sc-552180 | 200 µg Ab; 40 µg BP | $361.00 | |||
Josephin-3 (G-12) Neutralizing Peptide | sc-514821 P | 100 µg/0.5 ml | $69.00 |