JNK2 Antibody (A-7): sc-271133

JNK2 Antibody (A-7) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 391-424 at the C-terminus of human JNK2, a crucial stress-activated protein kinase involved in cellular stress responses. JNK2 mouse monoclonal antibody (A-7) recognizes an epitope within this C-terminal region and demonstrates broad species cross-reactivity, detecting JNK2 in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) applications. Anti-JNK2 monoclonal antibody (A-7) is available in non-conjugated form and multiple conjugated forms including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, offering versatility for diverse experimental needs. The complexity of JNK signaling emerges through sophisticated alternative splicing: three genes generate ten distinct isoforms, including five p46 variants (JNK1α1, JNK1β1, JNK2α1, JNK2β1, JNK3α1) and five p54 variants (JNK1α2, JNK1β2, JNK2α2, JNK2β2, JNK3β2). JNK2′s activation occurs under various stress conditions, leading to phosphorylation of transcription factors, particularly within the AP-1 complex containing c-Jun and c-Fos. JNK2 exhibits context-dependent functions in cancer biology: depending on the cellular environment, JNK2 can either promote or suppress tumor development in conditions including melanoma, squamous cell carcinoma, and basal cell carcinoma. Beyond cancer, JNK2 contributes to inflammatory conditions such as psoriasis and scleroderma. JNK2 participates in sophisticated protein interaction networks, forming regulatory complexes with upstream activators MKK4 and MKK7, and modulating apoptosis through interactions with p53 and Bcl-2 family members. Through these diverse cellular functions and complex regulatory mechanisms, JNK2 (A-7) antibody serves as a valuable tool for investigating stress responses, cancer biology, and inflammatory conditions.

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JNK2 Antibody (A-7) References:

1. Inhibition of c-Jun N-terminal kinase 2 expression suppresses growth and induces apoptosis of human tumor cells in a p53-dependent manner.  |  Potapova, O., et al. 2000. Mol Cell Biol. 20: 1713-22. PMID: 10669748
2. JNK is required for effector T-cell function but not for T-cell activation.  |  Dong, C., et al. 2000. Nature. 405: 91-4. PMID: 10811224
3. Isoforms of Jun kinase are differentially expressed and activated in human monocyte/macrophage (THP-1) cells.  |  Dreskin, SC., et al. 2001. J Immunol. 166: 5646-53. PMID: 11313405
4. Induced proliferation of human MRC-5 cells by nitrogen oxides via direct and indirect activation of MEKK1, JNK, and p38 signals.  |  Chou, FP., et al. 2002. Toxicol Appl Pharmacol. 181: 203-8. PMID: 12079429
5. Differential gene regulation by specific gain-of-function JNK1 proteins expressed in Swiss 3T3 fibroblasts.  |  Han, SY., et al. 2002. J Biol Chem. 277: 47167-74. PMID: 12354774
6. Roles of JNK-1 and p38 in selective induction of apoptosis by capsaicin in ras-transformed human breast epithelial cells.  |  Kang, HJ., et al. 2003. Int J Cancer. 103: 475-82. PMID: 12478662
7. JNK2 contains a specificity-determining region responsible for efficient c-Jun binding and phosphorylation.  |  Kallunki, T., et al. 1994. Genes Dev. 8: 2996-3007. PMID: 8001819
8. Defective T cell differentiation in the absence of Jnk1.  |  Dong, C., et al. 1998. Science. 282: 2092-5. PMID: 9851932

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-JNK2 Antibody (A-7) has 14 citations in a variety of scientific publications.

Primary antibodies like Anti-JNK2 Antibody (A-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

JNK2 Antibody (A-7) Specifications

Alternate Product Name: JNK2

Product Clone Name: A-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 391-424

Epitope Target: JNK2

Epitope Species: human

Product Reactivity/Detection: JNK2 p54 isoforms

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for JNK2 Antibody (A-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred p-JNK antibody is p-JNK Antibody (G-7): sc-6254.