JNK1/2 Antibody (D-9): sc-137019

JNK1/2 Antibody (D-9) is a mouse monoclonal IgG2b kappa light chain antibody raised against full-length human JNK1 p46 (amino acids 1-384), a stress-activated protein kinase essential for cellular stress responses. JNK1/2 mouse monoclonal antibody (D-9) recognizes both JNK1 and JNK2 isoforms and demonstrates broad species cross-reactivity, detecting p46 and p54 variants in mouse, rat, and human samples through Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) applications. JNK1 and JNK2 demonstrate remarkably context-dependent functions in disease, particularly in cancer biology. For example, JNK1 acts as a tumor promoter in hepatocellular carcinoma by enhancing cell growth and proliferation, yet surprisingly functions as a tumor suppressor in skin cancers. Similarly, JNK2 exhibits oncogenic properties across multiple cancer types, including myeloma, lung, and skin cancers, where JNK2 supports cancer cell survival. These diverse effects stem from JNK1/2′s participation in sophisticated protein interaction networks. Both kinases form regulatory complexes with key signaling proteins such as c-Jun, p53, and Bcl-2, thereby modulating critical cellular processes including apoptosis and inflammation. Through these interactions, JNK1/2 influence various transcription factors, most notably AP-1, creating intricate signaling cascades that control multiple cellular behaviors. When these finely balanced pathways become dysregulated, they can contribute to a spectrum of pathological conditions beyond cancer, including immune disorders and neurodegenerative diseases. Through these context-specific functions and complex interaction networks, JNK1/2 (D-9) antibody serves as a valuable tool for investigating disease mechanisms and developing targeted therapeutic strategies.

The preferred p-JNK antibody is p-JNK Antibody (G-7): sc-6254.