JNK1/2 Antibody (D-9): sc-137019

JNK1/2 Antibody (D-9) is a mouse monoclonal IgG2b kappa light chain antibody raised against full-length human JNK1 p46 (amino acids 1-384), a stress-activated protein kinase essential for cellular stress responses. JNK1/2 mouse monoclonal antibody (D-9) recognizes both JNK1 and JNK2 isoforms and demonstrates broad species cross-reactivity, detecting p46 and p54 variants in mouse, rat, and human samples through Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) applications. JNK1 and JNK2 demonstrate remarkably context-dependent functions in disease, particularly in cancer biology. For example, JNK1 acts as a tumor promoter in hepatocellular carcinoma by enhancing cell growth and proliferation, yet surprisingly functions as a tumor suppressor in skin cancers. Similarly, JNK2 exhibits oncogenic properties across multiple cancer types, including myeloma, lung, and skin cancers, where JNK2 supports cancer cell survival. These diverse effects stem from JNK1/2′s participation in sophisticated protein interaction networks. Both kinases form regulatory complexes with key signaling proteins such as c-Jun, p53, and Bcl-2, thereby modulating critical cellular processes including apoptosis and inflammation. Through these interactions, JNK1/2 influence various transcription factors, most notably AP-1, creating intricate signaling cascades that control multiple cellular behaviors. When these finely balanced pathways become dysregulated, they can contribute to a spectrum of pathological conditions beyond cancer, including immune disorders and neurodegenerative diseases. Through these context-specific functions and complex interaction networks, JNK1/2 (D-9) antibody serves as a valuable tool for investigating disease mechanisms and developing targeted therapeutic strategies.

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Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.

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JNK1/2 Antibody (D-9) References:

1. Cooperation between JNK1 and JNK2 in activation of p53 apoptotic pathway.  |  Oleinik, NV., et al. 2007. Oncogene. 26: 7222-30. PMID: 17525747
2. JNK1 and JNK2 differently regulate IL-12 production in THP-1 macrophage cells.  |  Utsugi, M., et al. 2010. Cytokine. 51: 127-31. PMID: 20483637
3. JNK1/2 regulates ER-mitochondrial Ca2+ cross-talk during IL-1β-mediated cell death in RINm5F and human primary β-cells.  |  Verma, G., et al. 2013. Mol Biol Cell. 24: 2058-71. PMID: 23615449
4. JNK1 and JNK2 regulate α-SMA in hepatic stellate cells during CCl4 -induced fibrosis in the rat liver.  |  Hong, IH., et al. 2013. Pathol Int. 63: 483-91. PMID: 24134609
5. JNK1/2 expression and modulation of STAT3 signaling in oral cancer.  |  Gkouveris, I., et al. 2016. Oncol Lett. 12: 699-706. PMID: 27347203
6. JNK1/2 and ERK1/2 provides vital clues about tumor recurrence and survival in hepatocellular carcinoma patients.  |  Gao, X., et al. 2018. Future Oncol. 14: 2471-2481. PMID: 29714074
7. Rigosertib-Activated JNK1/2 Eliminate Tumor Cells through p66Shc Activation.  |  Günther, JK., et al. 2020. Biology (Basel). 9: PMID: 32429320
8. Liquiritin Attenuates Angiotensin II-Induced Cardiomyocyte Hypertrophy via ATE1/TAK1-JNK1/2 Pathway.  |  Mo, J., et al. 2022. Evid Based Complement Alternat Med. 2022: 7861338. PMID: 35341136
9. The Involvement of Cx43 in JNK1/2-Mediated Endothelial Mechanotransduction and Human Plaque Progression.  |  Tauchi, M., et al. 2023. Int J Mol Sci. 24: PMID: 36674690
10. Glycyrrhetinic acid suppresses breast cancer metastasis by inhibiting M2-like macrophage polarization via activating JNK1/2 signaling.  |  Cheng, Y., et al. 2023. Phytomedicine. 114: 154757. PMID: 37011418
11. LCZ696 attenuates sepsis-induced liver dysfunction in rats; the role of oxidative stress, apoptosis, and JNK1/2-P38 signaling pathways.  |  Mohyeldin, RH., et al. 2023. Life Sci. 334: 122210. PMID: 37883863

The preferred p-JNK antibody is p-JNK Antibody (G-7): sc-6254.