JMY Antibody (G-11): sc-166030

JMY Antibody (G-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects JMY protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). JMY (G-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. JMY protein plays a crucial role as a nuclear cofactor for the p300 and CREB-binding protein (CBP) coactivators, which are essential for transcriptional activation of various genes, particularly in response to cellular stress. JMY enhances activation of p53 protein, a key regulator of cell cycle and apoptosis, by facilitating recruitment of p300/CBP complexes to p53 target genes. This interaction is vital because p53, when activated, induces transcription of genes critical for growth arrest and apoptosis, such as waf1/cip1, bax, mdm2, and gadd45. JMY′s ability to modulate p53 activity is further complicated by multiple isoforms generated through alternative splicing, which can differentially influence p53 responses, with some isoforms significantly enhancing p53-mediated transcriptional activation. Disruption of p300 and JMY complexes can inhibit p53-induced transcription of pro-apoptotic genes like bax, thereby blocking apoptosis and potentially contributing to tumorigenesis.

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JMY Antibody (G-11) References:

1. A novel cofactor for p300 that regulates the p53 response.  |  Shikama, N., et al. 1999. Mol Cell. 4: 365-76. PMID: 10518217
2. p300 requires its histone acetyltransferase activity and SRC-1 interaction domain to facilitate thyroid hormone receptor activation in chromatin.  |  Li, J., et al. 2000. Mol Cell Biol. 20: 2031-42. PMID: 10688650
3. The p53 response during DNA damage: impact of transcriptional cofactors.  |  Coutts, AS. and La Thangue, N. 2006. Biochem Soc Symp. 181-9. PMID: 16626298
4. Mdm2 targets the p53 transcription cofactor JMY for degradation.  |  Coutts, AS., et al. 2007. EMBO Rep. 8: 84-90. PMID: 17170761
5. The Role of JMY in p53 Regulation.  |  Adighibe, O. and Pezzella, F. 2018. Cancers (Basel). 10: PMID: 29857553
6. Regulation of JMY's actin nucleation activity by TTC5/STRAP and LC3 during autophagy.  |  Liu, X. and Klionsky, DJ. 2019. Autophagy. 15: 373-374. PMID: 30593260
7. p53-dependent DNA repair during the DNA damage response requires actin nucleation by JMY.  |  Rodriguez-Pastrana, I., et al. 2023. Cell Death Differ. 30: 1636-1647. PMID: 37142657
8. Binding and modulation of p53 by p300/CBP coactivators.  |  Lill, NL., et al. 1997. Nature. 387: 823-7. PMID: 9194565
9. Suppression of the p300-dependent mdm2 negative-feedback loop induces the p53 apoptotic function.  |  Thomas, A. and White, E. 1998. Genes Dev. 12: 1975-85. PMID: 9649502
10. Cell cycle regulation of the transcriptional coactivators p300 and CREB binding protein.  |  Snowden, AW. and Perkins, ND. 1998. Biochem Pharmacol. 55: 1947-54. PMID: 9714314
11. Role for p300 in stabilization of p53 in the response to DNA damage.  |  Yuan, ZM., et al. 1999. J Biol Chem. 274: 1883-6. PMID: 9890940
12. p53 sites acetylated in vitro by PCAF and p300 are acetylated in vivo in response to DNA damage.  |  Liu, L., et al. 1999. Mol Cell Biol. 19: 1202-9. PMID: 9891054

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-JMY Antibody (G-11) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-JMY Antibody (G-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

JMY Antibody (G-11) Specifications

Alternate Product Name: JMY

Product Clone Name: G-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-300

Epitope Target: JMY

Epitope Species: mouse

Product Reactivity/Detection: JMY

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for JMY Antibody (G-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin