JMJD5 Antibody (D-5): sc-377078
- JMJD5 Antibody (D-5) is a mouse monoclonal IgG2a κ JMJD5 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 215-253 within an internal region of JMJD5 of human origin
- JMJD5 Antibody (D-5) is recommended for detection of JMJD5 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine and porcine
- Anti-JMJD5 Antibody (D-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for JMJD5 Antibody (D-5). This work is in progress.
JMJD5 Antibody (D-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects JMJD5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). JMJD5 (D-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. JMJD5, known as jumonji domain containing 5, is primarily located in the nucleus and plays a crucial role as a histone lysine demethylase, essential for gene expression regulation and chromatin remodeling. JMJD5′s ability to demethylate histones is vital for maintaining proper cellular function and genomic stability, influencing DNA accessibility for transcription and repair processes. Notably, the C. elegans homolog of JMJD5 protects the genome from insertions and deletions, suggesting mammalian JMJD5 may function as a tumor suppressor. JMJD5 knockdown in mouse fibroblasts increases mutation rates and enhances tolerance to DNA-damaging agents, indicating involvement in DNA mismatch repair mechanisms. JMJD5 serves both epigenetic regulation and genomic integrity maintenance functions, making JMJD5 a critical target for cancer biology research and therapeutic development.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
JMJD5 Antibody (D-5) References:
- Jumonji is a nuclear protein that participates in the negative regulation of cell growth. | Toyoda, M., et al. 2000. Biochem Biophys Res Commun. 274: 332-6. PMID: 10913339
- Roles of JUMONJI in mouse embryonic development. | Jung, J., et al. 2005. Dev Dyn. 232: 21-32. PMID: 15580614
- Roles of jumonji and jumonji family genes in chromatin regulation and development. | Takeuchi, T., et al. 2006. Dev Dyn. 235: 2449-59. PMID: 16715513
- Tumor suppressor gene identification using retroviral insertional mutagenesis in Blm-deficient mice. | Suzuki, T., et al. 2006. EMBO J. 25: 3422-31. PMID: 16858412
- Histone lysine methyltransferases and demethylases in Plasmodium falciparum. | Cui, L., et al. 2008. Int J Parasitol. 38: 1083-97. PMID: 18299133
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
JMJD5 Antibody (D-5) | sc-377078 | 200 µg/ml | $322.00 | |||
JMJD5 Antibody (D-5) AC | sc-377078 AC | 500 µg/ml, 25% agarose | $424.00 | |||
JMJD5 Antibody (D-5) HRP | sc-377078 HRP | 200 µg/ml | $322.00 | |||
JMJD5 Antibody (D-5) FITC | sc-377078 FITC | 200 µg/ml | $336.00 | |||
JMJD5 Antibody (D-5) PE | sc-377078 PE | 200 µg/ml | $349.00 | |||
JMJD5 Antibody (D-5) Alexa Fluor® 488 | sc-377078 AF488 | 200 µg/ml | $364.00 | |||
JMJD5 Antibody (D-5) Alexa Fluor® 546 | sc-377078 AF546 | 200 µg/ml | $364.00 | |||
JMJD5 Antibody (D-5) Alexa Fluor® 594 | sc-377078 AF594 | 200 µg/ml | $364.00 | |||
JMJD5 Antibody (D-5) Alexa Fluor® 647 | sc-377078 AF647 | 200 µg/ml | $364.00 | |||
JMJD5 Antibody (D-5) Alexa Fluor® 680 | sc-377078 AF680 | 200 µg/ml | $364.00 | |||
JMJD5 Antibody (D-5) Alexa Fluor® 790 | sc-377078 AF790 | 200 µg/ml | $364.00 | |||
JMJD5 (D-5) Neutralizing Peptide | sc-377078 P | 100 µg/0.5 ml | $69.00 |