JIP-2 Antibody (A-9): sc-377490
- JIP-2 Antibody (A-9) is a mouse monoclonal IgG1 κ JIP-2 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 51-89 near the N-terminus of JIP-2 of human origin
- JIP-2 Antibody (A-9) is recommended for detection of JIP-2 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine and canine
- Anti-JIP-2 Antibody (A-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for JIP-2 Antibody (A-9) for WB applications. These reagents are now offered in bundles with JIP-2 Antibody (A-9) (see ordering information below).
JIP-2 Antibody (A-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects JIP-2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). JIP-2 (A-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. JIP-2 plays a crucial role in the c-Jun N-terminal kinase (JNK) signaling pathway, which is vital for cellular responses to stress, such as ultraviolet light exposure. JIP-2 acts as a scaffold protein that facilitates the interaction between JNKs and their substrates, thereby influencing the phosphorylation of key transcription factors like c-Jun. This interaction is essential for regulating gene expression in response to stress signals, making JIP-2 a significant player in cellular signaling and stress response mechanisms. Additionally, JIP-2 is known to interact with other proteins in the JNK signaling cascade, including JIP-1, which can modulate JNK activity and localization. JIP-2′s ability to coordinate these interactions underscores its importance in maintaining cellular homeostasis and responding to environmental challenges.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
JIP-2 Antibody (A-9) References:
- Molecular cloning of multiple splicing variants of JIP-1 preferentially expressed in brain. | Kim, IJ., et al. 1999. J Neurochem. 72: 1335-43. PMID: 10098834
- Oncoprotein-mediated signalling cascade stimulates c-Jun activity by phosphorylation of serines 63 and 73. | Smeal, T., et al. 1992. Mol Cell Biol. 12: 3507-13. PMID: 1630458
- Phosphorylation of c-jun mediated by MAP kinases. | Pulverer, BJ., et al. 1991. Nature. 353: 670-4. PMID: 1922387
- JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain. | Dérijard, B., et al. 1994. Cell. 76: 1025-37. PMID: 8137421
- The stress-activated protein kinase subfamily of c-Jun kinases. | Kyriakis, JM., et al. 1994. Nature. 369: 156-60. PMID: 8177321
- Transcriptional regulation by MAP kinases. | Davis, RJ. 1995. Mol Reprod Dev. 42: 459-67. PMID: 8607977
- A cytoplasmic inhibitor of the JNK signal transduction pathway. | Dickens, M., et al. 1997. Science. 277: 693-6. PMID: 9235893
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
JIP-2 Antibody (A-9) | sc-377490 | 200 µg/ml | $322.00 | |||
JIP-2 Antibody (A-9): m-IgG Fc BP-HRP Bundle | sc-530030 | 200 µg Ab; 10 µg BP | $361.00 | |||
JIP-2 Antibody (A-9): m-IgGκ BP-HRP Bundle | sc-523346 | 200 µg Ab, 40 µg BP | $361.00 | |||
JIP-2 Antibody (A-9): m-IgG1 BP-HRP Bundle | sc-543828 | 200 µg Ab; 20 µg BP | $361.00 | |||
JIP-2 Antibody (A-9) AC | sc-377490 AC | 500 µg/ml, 25% agarose | $424.00 | |||
JIP-2 Antibody (A-9) HRP | sc-377490 HRP | 200 µg/ml | $322.00 | |||
JIP-2 Antibody (A-9) FITC | sc-377490 FITC | 200 µg/ml | $336.00 | |||
JIP-2 Antibody (A-9) PE | sc-377490 PE | 200 µg/ml | $349.00 | |||
JIP-2 Antibody (A-9) Alexa Fluor® 488 | sc-377490 AF488 | 200 µg/ml | $364.00 | |||
JIP-2 Antibody (A-9) Alexa Fluor® 546 | sc-377490 AF546 | 200 µg/ml | $364.00 | |||
JIP-2 Antibody (A-9) Alexa Fluor® 594 | sc-377490 AF594 | 200 µg/ml | $364.00 | |||
JIP-2 Antibody (A-9) Alexa Fluor® 647 | sc-377490 AF647 | 200 µg/ml | $364.00 | |||
JIP-2 Antibody (A-9) Alexa Fluor® 680 | sc-377490 AF680 | 200 µg/ml | $364.00 | |||
JIP-2 Antibody (A-9) Alexa Fluor® 790 | sc-377490 AF790 | 200 µg/ml | $364.00 | |||
JIP-2 (A-9) Neutralizing Peptide | sc-377490 P | 100 µg/0.5 ml | $69.00 |