JAM4 Antibody (F-8): sc-390815
- JAM4 Antibody (F-8) is a mouse monoclonal IgG1 κ JAM4 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 242-263 near the C-terminus of JAM4 of mouse origin
- JAM4 Antibody (F-8) is recommended for detection of JAM4 of mouse and rat origin by WB, IP, IF and ELISA
- Anti-JAM4 Antibody (F-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for JAM4 Antibody (F-8) for WB applications. These reagents are now offered in bundles with JAM4 Antibody (F-8) (see ordering information below).
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JAM4 Antibody (F-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects JAM4 protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). JAM4 (F-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The junctional adhesion molecule (JAM) family, which includes JAM4, is part of the immunoglobulin superfamily and is primarily expressed in the tight junctions of epithelial and endothelial cells. JAM4 plays a crucial role in mediating calcium-independent homophilic cell adhesion, which is essential for maintaining the integrity of cell layers and regulating paracellular permeability. JAM4 interacts with MAGI-1 (membrane-associated guanylate kinase inverted-1), a scaffolding protein that is vital for organizing signaling complexes at the cell membrane. This interaction between JAM4 and MAGI-1 is particularly important in the kidney glomerulus and small intestine epithelial cells, where JAM4 helps regulate permeability and maintain proper barrier function. Understanding JAM4 and its interactions is critical for elucidating mechanisms of tissue integrity and immune response, making anti-JAM4 antibody (F-8) a valuable tool for researchers studying cell adhesion and junctional dynamics.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
JAM4 Antibody (F-8) References:
- Characterization and chromosomal localization of JAM-1, a platelet receptor for a stimulatory monoclonal antibody. | Naik, UP., et al. 2001. J Cell Sci. 114: 539-47. PMID: 11171323
- JAM4, a junctional cell adhesion molecule interacting with a tight junction protein, MAGI-1. | Hirabayashi, S., et al. 2003. Mol Cell Biol. 23: 4267-82. PMID: 12773569
- Roles of immunoglobulin-like loops of junctional cell adhesion molecule 4; involvement in the subcellular localization and the cell adhesion. | Tajima, M., et al. 2003. Genes Cells. 8: 759-68. PMID: 12940823
- JAM4 enhances hepatocyte growth factor-mediated branching and scattering of Madin-Darby canine kidney cells. | Mori, H., et al. 2004. Genes Cells. 9: 811-9. PMID: 15330858
- Altered expression of junctional adhesion molecule 4 in injured podocytes. | Harita, Y., et al. 2006. Am J Physiol Renal Physiol. 290: F335-44. PMID: 16118391
- MAGI-1 is a component of the glomerular slit diaphragm that is tightly associated with nephrin. | Hirabayashi, S., et al. 2005. Lab Invest. 85: 1528-43. PMID: 16155592
- Ligand-of-Numb protein X is an endocytic scaffold for junctional adhesion molecule 4. | Kansaku, A., et al. 2006. Oncogene. 25: 5071-84. PMID: 16832352
- A CTX family cell adhesion molecule, JAM4, is expressed in stem cell and progenitor cell populations of both male germ cell and hematopoietic cell lineages. | Nagamatsu, G., et al. 2006. Mol Cell Biol. 26: 8498-506. PMID: 16982697
- Junctional adhesion molecule, a novel member of the immunoglobulin superfamily that distributes at intercellular junctions and modulates monocyte transmigration. | Martìn-Padura, I., et al. 1998. J Cell Biol. 142: 117-27. PMID: 9660867
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
JAM4 Antibody (F-8) | sc-390815 | 200 µg/ml | $322.00 | |||
JAM4 Antibody (F-8): m-IgG Fc BP-HRP Bundle | sc-530272 | 200 µg Ab; 10 µg BP | $361.00 | |||
JAM4 Antibody (F-8): m-IgGκ BP-HRP Bundle | sc-523694 | 200 µg Ab, 40 µg BP | $361.00 | |||
JAM4 Antibody (F-8): m-IgG1 BP-HRP Bundle | sc-543967 | 200 µg Ab; 20 µg BP | $361.00 | |||
JAM4 Antibody (F-8) AC | sc-390815 AC | 500 µg/ml, 25% agarose | $424.00 | |||
JAM4 Antibody (F-8) HRP | sc-390815 HRP | 200 µg/ml | $322.00 | |||
JAM4 Antibody (F-8) FITC | sc-390815 FITC | 200 µg/ml | $336.00 | |||
JAM4 Antibody (F-8) PE | sc-390815 PE | 200 µg/ml | $349.00 | |||
JAM4 Antibody (F-8) Alexa Fluor® 488 | sc-390815 AF488 | 200 µg/ml | $364.00 | |||
JAM4 Antibody (F-8) Alexa Fluor® 546 | sc-390815 AF546 | 200 µg/ml | $364.00 | |||
JAM4 Antibody (F-8) Alexa Fluor® 594 | sc-390815 AF594 | 200 µg/ml | $364.00 | |||
JAM4 Antibody (F-8) Alexa Fluor® 647 | sc-390815 AF647 | 200 µg/ml | $364.00 | |||
JAM4 Antibody (F-8) Alexa Fluor® 680 | sc-390815 AF680 | 200 µg/ml | $364.00 | |||
JAM4 Antibody (F-8) Alexa Fluor® 790 | sc-390815 AF790 | 200 µg/ml | $364.00 | |||
JAM4 (F-8) Neutralizing Peptide | sc-390815 P | 100 µg/0.5 ml | $69.00 |