JAM-A Antibody (1H2A9): sc-53624

JAM-A Antibody (1H2A9) is a mouse monoclonal IgG2b kappa light chain antibody raised against a recombinant JAM fusion protein of human origin, specifically detecting JAM-A of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Junctional Adhesion Molecule A (JAM-A), also known as JAM-1 or F11 Receptor (F11R), is a member of the immunoglobulin superfamily involved in cell-cell adhesion at tight junctions of epithelial and endothelial cells. JAM-A functions to regulate paracellular permeability and maintain cell polarity, which are essential for tissue integrity and barrier function. JAM-A′s location at tight junctions allows for homophilic interactions, contributing to the formation and maintenance of these specialized cell-cell contacts. Structurally, JAM-A is a type I transmembrane protein with two extracellular immunoglobulin-like domains that facilitate adhesion and signaling. JAM-A′s cytoplasmic tail contains a PDZ-binding motif that interacts with scaffold proteins like ZO-1, AF-6, and PAR-3, linking to the actin cytoskeleton and intracellular signaling pathways. This structural arrangement is important for the organization of tight junctions and the modulation of leukocyte transmigration during inflammatory responses. Anti-JAM-A antibody (1H2A9) serves as a valuable tool for researchers studying cell adhesion, tight junction integrity, and immune cell trafficking, aiding in the understanding of mechanisms underlying vascular permeability and inflammation.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-JAM-A Antibody (1H2A9) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-JAM-A Antibody (1H2A9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

JAM-A Antibody (1H2A9) Specifications

Alternate Product Name: JAM-A

Product Clone Name: 1H2A9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: JAM

Epitope Species: human

Product Reactivity/Detection: JAM-A

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for JAM-A Antibody (1H2A9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred JAM-A antibody is JAM-A Antibody (J10.4): sc-53623.