JAM-A Antibody (J10.4) recommended for detection of JAM-A of mouse, rat and human origin by WB, IP, IF and FCM

JAM-A Antibody (J10.4): sc-53623

JAM-A Antibody (J10.4) is rated 4.8 out of 5 by 14.
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Datasheets

    • Anti-JAM-A Antibody (J10.4) is a mouse monoclonal IgG1 (kappa light chain) JAM-A antibody provided at 200 µg/ml
    • raised against recombinant JAM fusion protein of human origin
    • Anti-JAM-A Antibody (J10.4) is recommended for detection of JAM-A of mouse, rat and human origin by WB, IP, IF and FCM
    • Anti-JAM-A Antibody (J10.4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of JAM-A (J10.4): sc-53623.
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Can this antibody be used for staining mouse tissue?

Asked by: Anna99
Yes. We recommend using a directly conjugated antibody. JAM-A Antibody (J10.4): sc-53623 is available conjugated to HRP (sc-53623 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-53623 PE), fluorescein (sc-53623 FITC), Alexa Fluor® 488 (sc-53623 AF488) or Alexa Fluor® 647 (sc-53623 AF647), 200 µg/ml, for IF, IHC(P) and FCM.
Answered by: Technical Service 11
Date published: 2017-01-13

What is the recommended secondary for this mouse primary antibody?

Asked by: Anna99
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-01-13
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Rated 5 out of 5 by from good quality product This antibody is good quality give us specific bands,the stable quality can keep more than 1year for WB application.Always specific bands at 36kda.
Date published: 2018-01-27
Rated 4 out of 5 by from Works good on WB The antibody worked well on western blot on mouse tissue sample. It showed a clear band around 36kD. I also tried on IF but the result was not conclusive.
Date published: 2017-10-25
Rated 5 out of 5 by from specific/sharp bands detected This antibody was tested on human aortic endothelial cells post various treatments. It gives specific band with no background.
Date published: 2017-09-14
Rated 5 out of 5 by from super IF marker! It works very well for IF applications: IHC and ICC of human tissues
Date published: 2017-06-29
Rated 4 out of 5 by from Human gingival 3D tissue We got this antibody as a free sample. It works (1/50-1/400) in both frozen and paraffin embedded sections with some background.
Date published: 2017-04-25
Rated 5 out of 5 by from Outstanding product Clear, strong western data with little or no background; also great for IF staining applications
Date published: 2017-01-20
Rated 5 out of 5 by from good results for WB and IF This monoclonal shows good results for WB and IF staining on human samples.
Date published: 2017-01-11
Rated 4 out of 5 by from It works well in TM4 cells this antibody shows good immunofluorescence membrane staining in polyformaldehyde-fixed mouse TM4 cells.
Date published: 2016-12-29
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