JAM-A Antibody (J10.4): sc-53623

JAM-A Antibody (J10.4) is a mouse monoclonal IgG1 kappa light chain antibody raised against a recombinant JAM fusion protein of human origin, specifically reactive with JAM-A from mouse, rat, and human species as detected by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Junctional Adhesion Molecule A (JAM-A), also known as JAM-1 or F11 Receptor (F11R), is a member of the immunoglobulin superfamily prominently expressed at tight junctions of epithelial and endothelial cells. JAM-A interacts with various proteins such as ZO-1, AF-6, and PAR-3, which are PDZ domain-containing proteins crucial for tight junction assembly and maintenance. These interactions regulate cell polarity and paracellular permeability, essential for tissue homeostasis. Additionally, JAM-A interacts with integrins like LFA-1 (CD11a/CD18) on leukocytes, facilitating their transmigration across the endothelium during immune responses. JAM-A also associates with other tight junction proteins and signaling molecules, influencing cellular adhesion and motility. By studying these protein-protein interactions, researchers can gain insights into physiological processes such as inflammation, barrier function, and leukocyte extravasation. Anti-JAM-A antibody (J10.4) provides a specific and reliable tool for investigating JAM-A′s role in various biological contexts, making this reagent valuable for advancing our understanding of potential therapeutic targets.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

JAM-A Antibody (J10.4) References:

1. Cutting edge: combined treatment of TNF-alpha and IFN-gamma causes redistribution of junctional adhesion molecule in human endothelial cells.  |  Ozaki, H., et al. 1999. J Immunol. 163: 553-7. PMID: 10395639
2. Junctional adhesion molecule interacts with the PDZ domain-containing proteins AF-6 and ZO-1.  |  Ebnet, K., et al. 2000. J Biol Chem. 275: 27979-88. PMID: 10856295
3. Homophilic interaction of junctional adhesion molecule.  |  Bazzoni, G., et al. 2000. J Biol Chem. 275: 30970-6. PMID: 10913139
4. Junctional adhesion molecule (JAM) is phosphorylated by protein kinase C upon platelet activation.  |  Ozaki, H., et al. 2000. Biochem Biophys Res Commun. 276: 873-8. PMID: 11027562
5. The molecular organization of endothelial junctions and their functional role in vascular morphogenesis and permeability.  |  Dejana, E., et al. 2000. Int J Dev Biol. 44: 743-8. PMID: 11061439
6. Cloning of human junctional adhesion molecule 3 (JAM3) and its identification as the JAM2 counter-receptor.  |  Arrate, MP., et al. 2001. J Biol Chem. 276: 45826-32. PMID: 11590146
7. Vascular endothelial-junctional adhesion molecule (VE-JAM)/JAM 2 interacts with T, NK, and dendritic cells through JAM 3.  |  Liang, TW., et al. 2002. J Immunol. 168: 1618-26. PMID: 11823489
8. JAM2 interacts with alpha4beta1. Facilitation by JAM3.  |  Cunningham, SA., et al. 2002. J Biol Chem. 277: 27589-92. PMID: 12070135
9. Junctional adhesion molecule-2 (JAM-2) promotes lymphocyte transendothelial migration.  |  Johnson-Léger, CA., et al. 2002. Blood. 100: 2479-86. PMID: 12239159
10. Junctional adhesion molecule, a novel member of the immunoglobulin superfamily that distributes at intercellular junctions and modulates monocyte transmigration.  |  Martìn-Padura, I., et al. 1998. J Cell Biol. 142: 117-27. PMID: 9660867

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-JAM-A Antibody (J10.4) has 37 citations in a variety of scientific publications.

Primary antibodies like Anti-JAM-A Antibody (J10.4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

JAM-A Antibody (J10.4) Specifications

Alternate Product Name: JAM-A

Product Clone Name: J10.4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: JAM

Epitope Species: human

Product Reactivity/Detection: JAM-A

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 37 citations in various scientific publications

Available Conjugates/Forms for JAM-A Antibody (J10.4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin