ITF-2/TCF4 Antibody (C-8): sc-393407
- ITF-2/TCF4 Antibody (C-8) is a mouse monoclonal IgG2a κ ITF-2/TCF4 antibody, cited in 3 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 251-278 within an internal region of ITF-2 of human origin
- ITF-2/TCF4 Antibody (C-8) is recommended for detection of ITF-2 isoforms SEF2-1A and SEF2-1D of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ITF-2/TCF4 Antibody (C-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-393407 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ITF-2/TCF4 Antibody (C-8) for WB applications. These reagents are now offered in bundles with ITF-2/TCF4 Antibody (C-8) (see ordering information below).
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ITF-2 Antibody (C-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects ITF-2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ITF-2 (C-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Immunoglobulin transcription factor 2 (ITF-2), also known as transcription factor and SL3-3 enhancer factor 2 (SEF-2), is a basic helix-turn-helix transcription factor that plays a crucial role in regulating gene expression, particularly in muscle development. ITF-2 is primarily located in the nucleus, where ITF-2 binds to specific DNA sequences, such as the immunoglobulin enhancer Mu-E5/KE5-motif and the Ephrussi-box (E-box) element found in SSTR2-INR, thereby acting as a transcriptional activator for muscle-specific genes. ITF-2′s ability to form homo- or hetero-oligomers with other myogenic factors like myogenin and MyoD is essential for function, as these interactions can modulate the transcriptional activity of target genes, influencing muscle differentiation and development. ITF-2 expression in tissues such as the adult heart, brain, placenta, and skeletal muscle highlights importance in various biological processes, making anti-ITF-2 antibody (C-8) a valuable tool for researchers studying muscle biology and transcriptional regulation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ITF-2/TCF4 Antibody (C-8) References:
- Id-1, ITF-2, and Id-2 comprise a network of helix-loop-helix proteins that regulate mammary epithelial cell proliferation, differentiation, and apoptosis. | Parrinello, S., et al. 2001. J Biol Chem. 276: 39213-9. PMID: 11498533
- Helix-loop-helix transcriptional activators bind to a sequence in glucocorticoid response elements of retrovirus enhancers. | Corneliussen, B., et al. 1991. J Virol. 65: 6084-93. PMID: 1681116
- Heterodimers of myogenic helix-loop-helix regulatory factors and E12 bind a complex element governing myogenic induction of the avian cardiac alpha-actin promoter. | French, BA., et al. 1991. Mol Cell Biol. 11: 2439-50. PMID: 1850096
- Two distinct transcription factors that bind the immunoglobulin enhancer microE5/kappa 2 motif. | Henthorn, P., et al. 1990. Science. 247: 467-70. PMID: 2105528
- Sequence of the cDNA encoding ITF-2, a positive-acting transcription factor. | Henthorn, P., et al. 1990. Nucleic Acids Res. 18: 678. PMID: 2308860
- A splice variant of the ITF-2 transcript encodes a transcription factor that inhibits MyoD activity. | Skerjanc, IS., et al. 1996. J Biol Chem. 271: 3555-61. PMID: 8631961
- Physical and functional interactions between the transcriptional inhibitors Id3 and ITF-2b. Evidence toward a novel mechanism regulating muscle-specific gene expression. | Chen, B. and Lim, RW. 1997. J Biol Chem. 272: 2459-63. PMID: 8999959
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ITF-2/TCF4 Antibody (C-8) | sc-393407 | 200 µg/ml | $316.00 | |||
ITF-2 Antibody (C-8): m-IgG Fc BP-HRP Bundle | sc-530448 | 200 µg Ab; 10 µg BP | $354.00 | |||
ITF-2 Antibody (C-8): m-IgGκ BP-HRP Bundle | sc-523935 | 200 µg Ab, 40 µg BP | $354.00 | |||
ITF-2 Antibody (C-8): m-IgG2a BP-HRP Bundle | sc-547503 | 200 µg Ab; 10 µg BP | $354.00 | |||
ITF-2 Antibody (C-8) X | sc-393407 X | 200 µg/0.1 ml | $316.00 | |||
ITF-2 Antibody (C-8) AC | sc-393407 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ITF-2 Antibody (C-8) HRP | sc-393407 HRP | 200 µg/ml | $316.00 | |||
ITF-2 Antibody (C-8) FITC | sc-393407 FITC | 200 µg/ml | $330.00 | |||
ITF-2 Antibody (C-8) PE | sc-393407 PE | 200 µg/ml | $343.00 | |||
ITF-2 Antibody (C-8) Alexa Fluor® 488 | sc-393407 AF488 | 200 µg/ml | $357.00 | |||
ITF-2 Antibody (C-8) Alexa Fluor® 546 | sc-393407 AF546 | 200 µg/ml | $357.00 | |||
ITF-2 Antibody (C-8) Alexa Fluor® 594 | sc-393407 AF594 | 200 µg/ml | $357.00 | |||
ITF-2 Antibody (C-8) Alexa Fluor® 647 | sc-393407 AF647 | 200 µg/ml | $357.00 | |||
ITF-2 Antibody (C-8) Alexa Fluor® 680 | sc-393407 AF680 | 200 µg/ml | $357.00 | |||
ITF-2 Antibody (C-8) Alexa Fluor® 790 | sc-393407 AF790 | 200 µg/ml | $357.00 | |||
ITF-2 (C-8) Neutralizing Peptide | sc-393407 P | 100 µg/0.5 ml | $68.00 |