ISG15 Antibody (3C2): sc-130516
- ISG15 Antibody (3C2) is a Armenian hamster monoclonal IgG provided at 200 µg/ml
- raised against His-tagged recombinant ISG15 of mouse origin
- recommended for detection of ISG15 of mouse origin by WB, IP and IF
- See ISG15 (F-9): sc-166755 for ISG15 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ISG15 Antibody (3C2). This work is in progress.
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ISG15 Antibody (3C2) is an Armenian hamster monoclonal IgG antibody that detects ISG15 in mouse samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-ISG15 antibody (3C2) is raised against His-tagged recombinant ISG15 of mouse origin. ISG15, a 15 kDa protein, plays a crucial role in the immune response by functioning as a ubiquitin-like modifier, conjugating to various intracellular target proteins, including JAK1 and MAPK3/ERK1, through a unique enzymatic pathway that is distinct from that of ubiquitin. This modification is vital for regulating protein stability and activity, thereby influencing key signaling pathways involved in immune responses. ISG15 (3C2) antibody exhibits specific chemotactic activity towards neutrophils, activating them to release eosinophil chemotactic factors, which further underscores its importance in orchestrating immune cell interactions. ISG15 is predominantly located in the cytoplasm and is expressed in muscle, epithelial, neuronal, and lymphoid cells, highlighting its diverse roles in various tissues. Anti-ISG15 antibody (3C2) is an essential tool for researchers studying the intricate mechanisms of immune modulation and cellular signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ISG15 Antibody (3C2) References:
- ISG15, a Small Molecule with Huge Implications: Regulation of Mitochondrial Homeostasis. | Albert, M., et al. 2018. Viruses. 10: PMID: 30428561
- ISG15 and ISGylation is required for pancreatic cancer stem cell mitophagy and metabolic plasticity. | Alcalá, S., et al. 2020. Nat Commun. 11: 2682. PMID: 32472071
- ISG15 deficiency restricts HIV-1 infection. | Jurczyszak, D., et al. 2022. PLoS Pathog. 18: e1010405. PMID: 35333911
- Pressure overload induces ISG15 to facilitate adverse ventricular remodeling and promote heart failure. | Yerra, VG., et al. 2023. J Clin Invest. 133: PMID: 37115698
- ISG15: A link between innate immune signaling, DNA replication, and genome stability. | Wardlaw, CP. and Petrini, JHJ. 2023. Bioessays. 45: e2300042. PMID: 37147792
- Regulation of STING activity in DNA sensing by ISG15 modification. | Lin, C., et al. 2023. Cell Rep. 42: 113277. PMID: 37864791
- USP16 is an ISG15 cross-reactive deubiquitinase that targets pro-ISG15 and ISGylated proteins involved in metabolism. | Gan, J., et al. 2023. Proc Natl Acad Sci U S A. 120: e2315163120. PMID: 38055744
- ISG15 blocks cardiac glycolysis and ensures sufficient mitochondrial energy production during Coxsackievirus B3 infection. | Bredow, C., et al. 2024. Cardiovasc Res. 120: 644-657. PMID: 38309955
- ISG15/USP18/STAT2 is a molecular hub regulating IFN I-mediated control of Dengue and Zika virus replication. | Espada, CE., et al. 2024. Front Immunol. 15: 1331731. PMID: 38384473
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ISG15 Antibody (3C2) | sc-130516 | 200 µg/ml | $322.00 |