IRX5 CRISPR/Cas9 KO Plasmid (h): sc-404838
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IRX5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IRX5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IRX5 Antibody (IRX5C10G5): sc-81102
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IRX5 CRISPR/Cas9 KO Plasmid (h) | sc-404838 | 20 µg | $397.00 |
Overview
IRX5 encodes Iroquois homeobox protein 5, a sequence-specific transcription factor that regulates developmental patterning and cell fate decisions through control of gene expression programs. In human cells, IRX5 activity contributes to lineage specification, tissue morphogenesis, and maintenance of differentiated states by modulating transcriptional networks that intersect with developmental signaling pathways. Dysregulated IRX5 expression has been linked to altered proliferative and differentiation phenotypes, supporting its relevance to studies of tumor biology and developmental disorders. As a nuclear regulator, IRX5 is commonly investigated for its impact on chromatin-associated transcriptional control and downstream pathway wiring.
IRX5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the IRX5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the IRX5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the IRX5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IRX5 protein expression.
This CRISPR knockout system enables efficient generation of IRX5-deficient cell models for investigation of IRX5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting IRX5 exon(s) critical for IRX5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple IRX5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IRX5 CRISPR/Cas9 KO Plasmid (h) and IRX5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the IRX5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IRX5 HDR Plasmid (h) and IRX5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by IRX5 homology arms to support homology-directed repair at defined IRX5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.