Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IRS-4 CRISPR/Cas9 KO Plasmid (h): sc-402033
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IRS-4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IRS-4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IRS-4 Antibody (C-1): sc-373778
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IRS-4 CRISPR/Cas9 KO Plasmid (h) | sc-402033 | 20 µg | $397.00 |
Overview
Insulin receptor substrate 4 (IRS4) is a cytoplasmic adaptor protein that couples activated insulin and insulin-like growth factor receptors to downstream signaling networks. Through interactions that promote PI3K–AKT–mTOR and RAS–MAPK pathway activation, IRS-4 influences cellular proliferation, metabolism, and survival, with context-dependent roles distinct from other IRS family members. IRS4 expression and signaling alterations have been associated with dysregulated growth factor signaling in cancer biology and with broader metabolic signaling phenotypes, making it relevant for dissecting receptor tyrosine kinase crosstalk. Studying IRS-4 supports mechanistic investigation of signal transduction dynamics, feedback regulation, and pathway rewiring under oncogenic or metabolic stress conditions.
IRS-4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the IRS4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the IRS4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the IRS4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IRS-4 protein expression.
This CRISPR knockout system enables efficient generation of IRS4-deficient cell models for investigation of IRS-4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting IRS4 exon(s) critical for IRS-4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple IRS4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IRS-4 CRISPR/Cas9 KO Plasmid (h) and IRS-4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the IRS4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IRS-4 HDR Plasmid (h) and IRS-4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by IRS4 homology arms to support homology-directed repair at defined IRS4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.