IRP-2 Antibody (7H6): sc-33682

IRP-2 Antibody (7H6) is a mouse monoclonal IgG1 kappa light chain antibody that detects IRP-2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). IRP-2 Antibody (7H6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. IRP-2 plays a crucial role in iron metabolism, which is vital for maintaining cellular homeostasis. Located primarily in the cytoplasm, IRP-2 acts as a key regulator of iron homeostasis by binding to iron-responsive elements (IREs) in the mRNA of genes involved in iron uptake and storage. This binding can either inhibit translation or stabilize mRNA, thereby controlling the levels of proteins such as transferrin receptor and ferritin, which are essential for iron transport and storage, respectively. IRP-2′s ability to respond to fluctuations in iron levels and various stress signals, including nitrogen monoxide and cytokines, underscores its importance in cellular adaptation to changing iron availability and oxidative stress. Using IRP-2 (7H6) antibody in your research can provide valuable insights into the regulatory mechanisms of iron metabolism and their implications in various physiological and pathological conditions.

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IRP-2 Antibody (7H6) References:

1. S-nitrosylation of IRP2 regulates its stability via the ubiquitin-proteasome pathway.  |  Kim, S., et al. 2004. Mol Cell Biol. 24: 330-7. PMID: 14673166
2. Sodium nitroprusside promotes IRP2 degradation via an increase in intracellular iron and in the absence of S nitrosylation at C178.  |  Wang, J., et al. 2006. Mol Cell Biol. 26: 1948-54. PMID: 16479012
3. Expression of active iron regulatory factor from a full-length human cDNA by in vitro transcription/translation.  |  Hirling, H., et al. 1992. Nucleic Acids Res. 20: 33-9. PMID: 1738601
4. Iron-dependent degradation of IRP2 requires its C-terminal region and IRP structural integrity.  |  Wang, J., et al. 2008. BMC Mol Biol. 9: 15. PMID: 18226225
5. Homology between IRE-BP, a regulatory RNA-binding protein, aconitase, and isopropylmalate isomerase.  |  Hentze, MW. and Argos, P. 1991. Nucleic Acids Res. 19: 1739-40. PMID: 1903202
6. A regulated RNA binding protein also possesses aconitase activity.  |  Kaptain, S., et al. 1991. Proc Natl Acad Sci U S A. 88: 10109-13. PMID: 1946430
7. Tumorigenic properties of iron regulatory protein 2 (IRP2) mediated by its specific 73-amino acids insert.  |  Maffettone, C., et al. 2010. PLoS One. 5: e10163. PMID: 20405006
8. Cloning of the cDNA encoding an RNA regulatory protein--the human iron-responsive element-binding protein.  |  Rouault, TA., et al. 1990. Proc Natl Acad Sci U S A. 87: 7958-62. PMID: 2172968
9. IRP2-Hif1α/Hif2α signaling: a novel mechanism of metabolic switch from aerobic glycolysis to oxidative phosphorylation.  |  Zhu, L., et al. 2020. Acta Biochim Biophys Sin (Shanghai). 52: 1175-1177. PMID: 32813008
10. Nadolol Attenuates Brain Cell Ferroptosis in Ischemic Stroke Rats by Targeting the HOIL-1/IRP2 Pathway.  |  Yang, XY., et al. 2024. CNS Neurol Disord Drug Targets.. PMID: 39252618
11. Chromosomal irp2 gene in Yersinia: distribution, expression, deletion and impact on virulence.  |  de Almeida, AM., et al. 1993. Microb Pathog. 14: 9-21. PMID: 8321119
12. The impact of oxidative stress on eukaryotic iron metabolism.  |  Rouault, TA. and Klausner, RD. 1996. EXS. 77: 183-97. PMID: 8856975

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IRP-2 Antibody (7H6) has 54 citations in a variety of scientific publications.

Primary antibodies like Anti-IRP-2 Antibody (7H6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IRP-2 Antibody (7H6) Specifications

Alternate Product Name: IRP-2

Product Clone Name: 7H6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: IRP-2 (iron regulatory protein-2)

Epitope Species: human

Product Reactivity/Detection: IRP-2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 54 citations in various scientific publications

Available Conjugates/Forms for IRP-2 Antibody (7H6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin