IRF-5 Antibody (C-6) recommended for detection of IRF-5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

IRF-5 Antibody (C-6): sc-390364

IRF-5 Antibody (C-6) is rated 5.0 out of 5 by 4.
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Datasheets

    • Anti-IRF-5 Antibody (C-6) is a mouse monoclonal IgG2b κ IRF-5 antibody, cited in 2 publications, provided at 200 µg/ml
    • raised against amino acids 443-498 mapping at the C-terminus of IRF-5 of human origin
    • Anti-IRF-5 Antibody (C-6) is recommended for detection of IRF-5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-IRF-5 Antibody (C-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IRF-5 (C-6): sc-390364.

I am using IRF-5 (C-6): sc-390364 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from nice clarity and signal intensity from IF We used this antibody in Immunoflourescence staining and were able to take nice images of the HeLa cells with the clear and intense signal
Date published: 2017-02-03
Rated 5 out of 5 by from Beautiful IF staining with methanol Beautiful IF staining with methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-05
Rated 5 out of 5 by from Robust nuclear and cytoplasmic staining in Robust nuclear and cytoplasmic staining in paraffin-embedded human appendix tissue. -SCBT QC
Date published: 2015-02-16
Rated 5 out of 5 by from Great mono Great mono! Works well for Western with SR and Ramos whole cell lysates. -SCBT QC
Date published: 2013-12-14
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