IRF-2 Antibody (G-10) recommended for detection of IRF-2 of mouse, rat and human origin by WB, IP, IF and ELISA

IRF-2 Antibody (G-10): sc-374327

IRF-2 Antibody (G-10) is rated 4.5 out of 5 by 2.
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Datasheets

    • Anti-IRF-2 Antibody (G-10) is a mouse monoclonal IgG1 κ IRF-2 antibody, cited in 2 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 317-346 at the C-terminus of IRF-2 of human origin
    • Anti-IRF-2 Antibody (G-10) is recommended for detection of IRF-2 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine and bovine
    • TransCruz reagent for ChIP application (sc-374327 X, 200 µg/0.1 ml)
    • Anti-IRF-2 Antibody (G-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IRF-2 (G-10): sc-374327.

Do you have western blot image of IRF2 in THP1 cells ? I purchased your antibody and tested it. I see two bands - the stronger band has the size that is about 40 kD and weaker band (about 1/5th in signal strength compared to stronger band) at ~50 kD.

Asked by: Lodoe
Thank you for your question. We do not currently have any WB data in THP1 cells. Please reach out to our Technical Service Department for assistance in troubleshooting this antibody. We are happy to help!
Answered by: Tech Service
Date published: 2019-10-02

What application is the blocking peptide sc-374327 P appropriate for?

Asked by: AbPolly
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from Ottimo funzionamento Ho comprato l'articolo circa un mese fa e l'ho impiegato in saggi di western blot con ottimi risultati.
Date published: 2018-05-06
Rated 5 out of 5 by from Great antibody for Western Great antibody for Western, strong band detected in mouse IRF-2 transfected 293T lysate. -SCBT QC
Date published: 2014-02-03
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