IRE1 alpha Antibody (B-12) recommended for detection of IRE1α of mouse, rat and human origin by WB, IP, IF and ELISA

IRE1α Antibody (B-12): sc-390960

IRE1α Antibody (B-12) is rated 4.5 out of 5 by 22.
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Datasheets

    • Anti-IRE1α Antibody (B-12) is a mouse monoclonal IgG1 (kappa light chain) IRE1α antibody provided at 200 µg/ml
    • raised against amino acids 371-560 of IRE1α of human origin
    • Anti-IRE1 alpha Antibody (B-12) is recommended for detection of IRE1α of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-IRE1 alpha Antibody (B-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IRE1α (B-12): sc-390960.

Can this antibody detect both phosphorylated and non-phosphorylated forms of IRE1-α?

Asked by: xiaotuichuchu
Thank you for your question. IRE1α Antibody (B-12): sc-390960 was aised against amino acids 371-560 of IRE1α of human origin. It recognizes both the phosphorylated and the non-phosphorylated IRE1α.
Answered by: Tech Support Europe
Date published: 2019-07-10

Does this antibody recognize Ire1 from Drosophila?

Asked by: FC 2018
Thank you for your question. IRE1α Antibody (B-12): sc-390960 is recommended for detection of IRE1α of mouse, rat and human origin by WB, IP, IF and ELISA. There is currently no data on the antibody’s reactivity in Drosophila. We have not received any customer feedback indicating reactivity in other species than mouse, rat, or human. Please contact our technical service for further information or to arrange for a free sample of the antibody to test whether it would be suitable for your application.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

What is the exact epitope recognized by IRE1α Antibody (B-12): sc-390960?

Asked by: cjMara
IRE1α Antibody (B-12) is a mouse monoclonal raised against amino acids 371-560 of IRE1α of human origin. The exact epitope has not been determined.
Answered by: Technical Support 13
Date published: 2017-01-09
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Rated 4 out of 5 by from clean but weak We got this as a sample. We tried it with mouse cortical neuron cultures lysate. The signal was ok but somewhat weak in our opinion.
Date published: 2018-11-17
Rated 5 out of 5 by from 抗体效果非常好,条带漂亮 我一个月前购买了此产品,很高兴,使用了非常的好,有需要这个指标的童鞋们不要犹豫了,实验结果非常完美。
Date published: 2018-07-17
Rated 5 out of 5 by from Really nice band! We checked the antibody in MDCK cells (canine). We used the antibody in 1:1.000 dilution in bovine serum albumin (1% in TBST), and the secondary antibody in 1:10.0000 dilution. We got a great band, with the expected molecular weight.
Date published: 2018-04-09
Rated 5 out of 5 by from Great feature The sample IRE1α (B-12): sc-390960 works perfectly.
Date published: 2017-11-04
Rated 4 out of 5 by from works fine for WB on murine heart samples loaded 30 µg protein, blocked 1h with 3% BSA, incubation of the ab over night at 4°C
Date published: 2017-10-31
Rated 3 out of 5 by from a clear band I used this antibody with a dilution of 1:200 and got a clear band.
Date published: 2017-10-23
Rated 4 out of 5 by from I will try it again there is no band in western blot test,with 1: 500 dilution.
Date published: 2017-10-18
Rated 5 out of 5 by from 可以 我做的大鼠样品,可以跑出一个明晰的条带,在100kd以上,分子量是对的,特异性也挺好,值得推广及购买!
Date published: 2017-10-17
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