IRE1α Antibody (B-12): sc-390960

IRE1α Antibody (B-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects IRE1 alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IRE1α (B-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. IRE1 alpha protein plays a crucial role in the unfolded protein response (UPR), a cellular stress response activated when misfolded or unfolded proteins accumulate in the endoplasmic reticulum (ER). This response is vital for maintaining cellular homeostasis and preventing apoptosis under stress conditions. IRE1α, along with IRE1β homolog, is localized in the ER lumen and features a transmembrane domain spanning the ER membrane, as well as a cytosolic Ser/Thr kinase domain. Upon activation, IRE1α undergoes oligomerization and trans-phosphorylation, which enhances kinase activity and leads to UPR-targeted gene transcription. This process is essential for proper protein folding and ER function restoration. Additionally, IRE1α is involved in Jun N-Terminal Kinase (JNK) phosphorylation, thereby activating the cellular MAP kinase pathway, which further underscores IRE1α′s importance in cellular signaling and stress response mechanisms.

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IRE1α Antibody (B-12) References:

1. Coupling of stress in the ER to activation of JNK protein kinases by transmembrane protein kinase IRE1.  |  Urano, F., et al. 2000. Science. 287: 664-6. PMID: 10650002
2. The metabolic ER stress sensor IRE1α suppresses alternative activation of macrophages and impairs energy expenditure in obesity.  |  Shan, B., et al. 2017. Nat Immunol. 18: 519-529. PMID: 28346409
3. Inhibition of neutrophil extracellular trap formation attenuates NLRP1-dependent neuronal pyroptosis via STING/IRE1α pathway after traumatic brain injury in mice.  |  Cao, Y., et al. 2023. Front Immunol. 14: 1125759. PMID: 37143681
4. HIF1α-dependent hypoxia response in myeloid cells requires IRE1α.  |  Mawambo, G., et al. 2023. J Neuroinflammation. 20: 145. PMID: 37344842
5. Loss of DDRGK1 impairs IRE1α UFMylation in spondyloepiphyseal dysplasia.  |  Yang, X., et al. 2023. Int J Biol Sci. 19: 4709-4725. PMID: 37781516
6. Targeting IRE1α reprograms the tumor microenvironment and enhances anti-tumor immunity in prostate cancer.  |  Unal, B., et al. 2024. Nat Commun. 15: 8895. PMID: 39406723
7. Transcriptional induction of genes encoding endoplasmic reticulum resident proteins requires a transmembrane protein kinase.  |  Cox, JS., et al. 1993. Cell. 73: 1197-206. PMID: 8513503
8. Gene induction in response to unfolded protein in the endoplasmic reticulum is mediated through Ire1p kinase interaction with a transcriptional coactivator complex containing Ada5p.  |  Welihinda, AA., et al. 1997. Proc Natl Acad Sci U S A. 94: 4289-94. PMID: 9113982
9. A pathway distinct from the mammalian unfolded protein response regulates expression of endoplasmic reticulum chaperones in non-stressed cells.  |  Brewer, JW., et al. 1997. EMBO J. 16: 7207-16. PMID: 9384597
10. A stress response pathway from the endoplasmic reticulum to the nucleus requires a novel bifunctional protein kinase/endoribonuclease (Ire1p) in mammalian cells.  |  Tirasophon, W., et al. 1998. Genes Dev. 12: 1812-24. PMID: 9637683
11. Cloning of mammalian Ire1 reveals diversity in the ER stress responses.  |  Wang, XZ., et al. 1998. EMBO J. 17: 5708-17. PMID: 9755171
12. Protein translation and folding are coupled by an endoplasmic-reticulum-resident kinase.  |  Harding, HP., et al. 1999. Nature. 397: 271-4. PMID: 9930704

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IRE1α Antibody (B-12) has 68 citations in a variety of scientific publications.

Primary antibodies like Anti-IRE1α Antibody (B-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IRE1α Antibody (B-12) Specifications

Alternate Product Name: IRE1α

Product Clone Name: B-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 371-560

Epitope Target: IRE1α

Epitope Species: human

Product Reactivity/Detection: IRE1α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 68 citations in various scientific publications

Available Conjugates/Forms for IRE1α Antibody (B-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin