IRAP Antibody (F-5): sc-365300

IRAP Antibody (F-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects IRAP in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). IRAP (F-5) antibody is available in both non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. IRAP, also known as Insulin-responsive aminopeptidase, LNPEP, OTase, or P-LAP, is a 1,025 amino acid enzyme predominantly expressed in the placenta, heart, kidney, and small intestine, with lower expression levels found in neuronal cells of the brain, skeletal muscle, spleen, liver, testis, and colon. IRAP belongs to the peptidase M1 family and plays a crucial role in degrading bioactive peptides, including hormones such as oxytocin, vasopressin, and angiotensin III, which are vital for maintaining homeostasis during pregnancy and regulating various physiological processes. IRAP influences glucose uptake in cells through trafficking of the insulin-responsive glucose transporter Glut4, making IRAP essential for metabolic regulation. IRAP also interacts with Tankyrase-1 and Tankyrase-2, significant signaling targets of extracellular signal-regulated kinase (ERK) in the Golgi apparatus, highlighting IRAP′s role in cellular signaling pathways. Three isoforms exist due to alternative splicing, underscoring IRAP′s complexity and functional diversity in various biological contexts.

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IRAP Antibody (F-5) References:

1. Tankyrase is a golgi-associated mitogen-activated protein kinase substrate that interacts with IRAP in GLUT4 vesicles.  |  Chi, NW. and Lodish, HF. 2000. J Biol Chem. 275: 38437-44. PMID: 10988299
2. Tankyrase-2 oligomerizes with tankyrase-1 and binds to both TRF1 (telomere-repeat-binding factor 1) and IRAP (insulin-responsive aminopeptidase).  |  Sbodio, JI., et al. 2002. Biochem J. 361: 451-9. PMID: 11802774
3. Role of the insulin-regulated aminopeptidase IRAP in insulin action and diabetes.  |  Keller, SR. 2004. Biol Pharm Bull. 27: 761-4. PMID: 15187412
4. Insulin-stimulated exocytosis of GLUT4 is enhanced by IRAP and its partner tankyrase.  |  Yeh, TY., et al. 2007. Biochem J. 402: 279-90. PMID: 17059388
5. Sub-cellular localization of insulin-regulated membrane aminopeptidase, IRAP to vesicles in neurons.  |  Fernando, RN., et al. 2007. J Neurochem. 102: 967-76. PMID: 17504262
6. Effects of ligands or substrate of insulin-regulated aminopeptidase (IRAP) on trophoblast invasion.  |  Cohen, M., et al. 2008. J Pept Sci. 14: 883-7. PMID: 18275097
7. Recycling of IRAP from the plasma membrane back to the insulin-responsive compartment requires the Q-SNARE syntaxin 6 but not the GGA clathrin adaptors.  |  Watson, RT., et al. 2008. J Cell Sci. 121: 1243-51. PMID: 18388312
8. The insulin-regulated aminopeptidase IRAP is colocalised with GLUT4 in the mouse hippocampus--potential role in modulation of glucose uptake in neurones?  |  Fernando, RN., et al. 2008. Eur J Neurosci. 28: 588-98. PMID: 18702730
9. Development of cognitive enhancers based on inhibition of insulin-regulated aminopeptidase.  |  Chai, SY., et al. 2008. BMC Neurosci. 9 Suppl 2: S14. PMID: 19090987
10. IRAP and AT(1) receptor mediated effects of angiotensin IV.  |  Dupont, AG., et al. 2009. J Intern Med. 265: 401-3; author reply 404-5. PMID: 19207373
11. IRAP Endosomes Control Phagosomal Maturation in Dendritic Cells.  |  Weimershaus, M., et al. 2020. Front Cell Dev Biol. 8: 585713. PMID: 33425891
12. The emerging multifunctional roles of ERAP1, ERAP2 and IRAP between antigen processing and renin-angiotensin system modulation.  |  Mattorre, B., et al. 2022. Front Immunol. 13: 1002375. PMID: 36203608

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IRAP Antibody (F-5) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-IRAP Antibody (F-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IRAP Antibody (F-5) Specifications

Alternate Product Name: IRAP

Product Clone Name: F-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 15-144

Epitope Target: IRAP

Epitope Species: human

Product Reactivity/Detection: IRAP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for IRAP Antibody (F-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin